IL-1B基因多态性、幽门螺旋杆菌感染和慢性萎缩性胃炎关系的研究

目的通过研究IL-1B-511基因多态性对幽门螺旋杆菌(Helicobacter pylori,H.pylori)感染后胃黏膜萎缩、慢性萎缩性胃炎的影响,探讨IL-1B基因多态性与胃癌发生的可能机制。方法(1)采用PCR-限制性长度片段多态性(RFLP)分析法检测胃癌低发区广东省普通人群192例的基因型;(2)采用酶联免疫吸附法(ELISA)检测上述人群的Hp感染率、胃蛋白酶原I(PGl)、胃蛋白酶原Ⅱ(PGII)和胃泌素(Gastrin)的浓度。结果Hp阳性者PGI/PGII显著低于Hp阴性者(P<0.01),Hp阳性的IL-1B-511T/T基因型者PGI/PGII比值显著低于C/C和T/T基因型者(P均<0.05)。血清胃泌素浓度与IL-1B-511的基因型没有明确的关系(P>0.05)。结论在胃癌低发区,IL-1B-511基因型可能增加感染H.pylori后胃黏膜萎缩、慢性萎缩性胃炎发生和发展的危险性。

Study of the Relation of Interleukin-1B Gene Polymorphism and H. Pylori Infection on Chronic Atrophic Gastritis

Objective: To investigate interleukin-lB gene polymorphism effect the mucosal atrophy and chronic atrophic gastritis with H.pylori infection.Methods: (1) IL-1B gene polymorphisms of common people were analyzed by PCR-RFLP.(2)The H.pylori infection rate,PG I,PGII and gastrin were examined using ELISA or RIA.Results: In low prevalent region people with H.pylori infection have higher PG I level,but lower PG I/PG II ratio,compared with individuals without H.pylori infection.IL-1B-511 T/T genotype individuals with H.pylori infection have lower PG I/PGII ratio than the C/C and T/T genotype H.pylori individuals,the gastrin level was similar among each genotype in IL-1B-511 locus no matter whether H.pylori infected or not.Conclusion: In low-prevalent region,IL-1B-511 T/Tgenotype was a risk factor for mucosal atrophy and atrophic gastritis after H.pylori infection.