延胡索碱预处理对缺血再灌注心肌梗死范围的影响

目的 观察延胡索碱预处理对大鼠心肌是否有缩小心肌梗死范围的干预作用.方法 将清洁级成年SD大鼠30只随机分为延胡索碱高、中、低剂量预处理组和经典缺血预处理组、模型组、假手术组6组(n=5),冠状动脉原位结扎,经主动脉逆行灌注2％伊文蓝,分离非缺血区和缺血区及梗死区和未梗死区,比较6组大鼠心肌缺血面积和心肌梗死面积.结果 ①心肌缺血面积:假手术组未见心肌缺血发生;与模型组比较,经典预处理组和延胡索碱中剂量组、高剂量组心肌缺血面积均有减少(44.6±5.7)％、(46.4±7.2)％、(45.7±5.7)％比(50.4±3.3)％],差异均有统计学意义(P＜0.05或P＜0.01);与经典预处理组比较,延胡索低剂量组心肌缺血面积大(48.9±4.4)％比(44.6±5.7)％],差异有统计学意义(P＜0.05),中剂量组、高剂量组差异无统计学意义(P＞0.05).②心肌梗死面积:假手术组未见心肌梗死发生.与模型组比较,经典预处理组和延胡索碱高、中、低剂量组梗死面积均缩小(8.9±3.4)％、(8.5±1.8)％、( 10.1±2.7)％、(14.2±5.2)％比(50.4±3.3)％],差异均有统计学意义(均P＜0.01);与经典预处理组比较,延胡索碱低剂量组心肌梗死面积较大(14.2±5.2)％比(8.9±3.4)％,P＜0.01],高、中剂量组心肌梗死面积与其相当,差异无统计学意义(P＞0.05).结论 延胡索碱预处理对缺血再灌注心肌的保护效应与缺血预处理极为相似,这一保护效应的作用机制很可能类似于缺血预处理,即延胡索碱可能模拟缺血预处理,激活机体内源性保护机制,产生预处理效应.

Effect of corydalis alkaloid pre-processing on ischemia-reperfusion myocardial infarction range

Objective To observe whether corydalis alkaloid preprocessing can narrow myocardial infarction range of rat＇s myoeardium. Methods All 30 adult rats with clean level were randomly divided into six groups （ high, middle and low doses of eorydalis alkaloid groups, classical isehemia pre-processing group, model group and sham operation group, n = 5 each group）. The heart was separated from body, and was ligated and retrogradely poured into 2% Evan＇s blue through aorta, and then myocardial non-isehemic area and ischemic area, infracted area and ischemia non-infraeted area were separated. The myocardial ischemia area and myocardial infarction area were compared among six groups. Results ①There was no myocardial ischemia in the sham operation group. Compared with the model group, myocardial ischemia area in classical ischemia pre-processing group, and middle, high doses of corydalis alkaloid groups all reduced （44.6±5.7）, （46.4±7.2）, （45.7±5.7）% vs （50.4±3.3）% ] （P 〈0.01 or P 〈 0.05 ）. Compared with classical ischemia pre-processing group, there were no significant differences （P 〉 0.05） on myocardial ischemia area in the high and middle doses groups except the low dose of corydalis alkaloid group （48.9±4.4）% vs （44.6±5.7）%, P 〈0.05]. ②There was no myocardial infarction in sham operation group. Compared with the model group, the infarction size of both classical ischemia pre-processing group and high, middle and low doses levels of corydalis alkaloid groups reduced （8.9±3.4）, （8.5 ±1.8）（ 10. 1±2.7）, （14. 2±5.2） % vs （50.4±3.3） % ] （ all P 〈 0.01 ）. Compared with the classical ischemia pre-processing group, there was no significant difference on myocardial infarct size in high and middle doses groups except the low dose of corydalis alkaloid group（14.2±5.2）% vs （8.9±3.4）%] （P〈0.01）. Conclusions There are great similarities be- tween the protection effect of corydalis alkaloid pre-processing and ischemia preconditioning. Corydalis alkaloid may simulate ischemia preconditioning, motivate endogenous protection system and produce the pre-processing effect.