维吾尔族妇女子宫颈病变中抑癌基因和人乳头瘤状病毒表达相关性研究

目的 探讨抑癌基因人第10号染色体缺失的磷酸酶及张力蛋白同源的基因(PTEN)、人乳头瘤病毒(HPV)与新疆维吾尔族妇女子宫颈病变的相关关系.方法 选取维吾尔族妇女正常或炎症的子宫颈组织标本30例为对照组,宫颈病理标本按病变程度及病理学诊断分为宫颈上皮内瘤样病变(CIN)Ⅰ组、CINⅡ～Ⅲ组各30例、宫颈鳞癌组织30例子宫颈鳞癌组采用免疫组化SP法检测PTEN蛋白表达,采用聚合酶链反应(PCR)检测HPV阳性率.结果 PTEN的蛋白表达率在正常或炎症的子宫颈组织、CIN Ⅰ、CINⅡ～Ⅲ、宫颈鳞癌组织中分别为83.3％ 、73.3％、56.7％、23.3％,子宫颈鳞癌组阳性表达率明显减少,与前3组比较差异有统计学意义(P＜0.05).4组HPV阳性率分别为43.3％、56.7％、76.7％、93.3％,HPV阳性率和子宫颈病变程度呈正相关(P＜0.05).结论 新疆维吾尔族妇女子宫颈病变组织中PTEN蛋白水平表达减少,与其宫颈病变呈负相关关系；是子宫颈组织恶变的信号.HPV感染与子宫颈病变的严重程度呈正相关,HPV感染在子宫颈病变的发展过程中起到了极大的促进作用,是子宫颈癌致病因素之一.

The correlation of phosphataseand tensin homdogy deletedon chromosome ten expression and human papilloma virus infection with the development of cervical lesions in Uygur women

Objective To investigate the correlation of phosphataseand tensin homdogy deletedon chromosome ten（PTEN） expression and human papilloma virus （HPV） infection in the cervical lesions of Uygur women. Methods Thirty cases of tissues for each of normal or inflammatory cervical lesion （ control）, cervical intraepithe- lial neoplasia（CIN） I , CIN Ⅱ and Ⅲ, cervical squamous carcinoma were collected from Uygur women for immu- nohistochemical detection of PTEN expression. HPV infection was confirmed by polymerase chain reaction amplification. Results PTEN was expressed in 83.3% , 73.3% , 56. 7% and 23. 3% of the normal or inflammatory cervical tissues, CIN I s, CIN Ⅱ～Ⅲ s and cervical squamous cell carcinoma tissues, respectively. In cervical squamous carcinoma tissue, expression of PTEN was much lower than that of others lesion groups. HPV was positive in 43.3% , 56.7%, 76. 7% and 93.3% of the normal or inflammatory cervical tissues, CIN I s, CIN Ⅱ～Ⅲ s and cervical squamous cell carcinoma tissues, respectively. HPV infection was positively correlated with the severity of cervical lesions （P 〈 0. 05 ）. Conclusion PTEN protein is down-regulated in the cervical lesions of Uygur women and is negatively coreelated with the severity of cervical lesions. HPV infection is negatively correlated with, as one of the promoting factors, the development of cervical transformation.