羟苯磺酸钙对去甲斑蝥素致血管内皮细胞毒性拮抗作用的研究

目的 探讨羟苯磺酸钙对去甲斑蝥素致血管内皮细胞损伤的影响,并测定羟苯磺酸钙的有效拮抗作用浓度.方法 将体外培养人脐静脉内皮细胞系完全随机分为对照组、毒性组和干预组.对照组用DMEM培养基培养48 h；毒性组用含去甲斑蝥素浓度为50μmol/L的DEME培养基培养48 h；干预组分为7组,分别加入含羟苯磺酸钙5、10、20、60、80、100和200 mmoL/L的DMEM培养基中再培养46h.测定细胞存活率.结果 干预组细胞存活率随着羟苯磺酸钙剂量的增加而升高,羟苯磺酸钙剂量达80 mmol/L时,细胞存活率明显升高.四甲基偶氮唑盐微量酶反应比色法测定的干预组(5 mmol/L)细胞存活率与毒性组相近,差异无统计学意义(P＞0.05),干预组(200 mmol/L)细胞存活率高于毒性组,组间比较差异有统计学意义[(69.4±3.8)％比(49.5±1.0)％,P＜0.05].结论 去甲斑瞀素抑制内皮细胞增殖,降低细胞存活率,当羟苯磺酸钙的浓度为80 mmol/L时,对去甲斑蝥素的毒性具有明显拮抗作用,能有效保护血管内皮细胞.

Study on the antagonism of calcium dobesilate regarding the vascular endothelial cells toxicity of nor cantharidin

Objective To investigate the antagonism of calcium dobesilate in vascular endothelial cells （VEC） injury by Nor cantharidin and to determine effective concentration of calcium dobesilate in the antagonism. Methods Human Umbilical Vein Endothelial Cells （HUVEC） were cultured in vitro and randomly divided into three groups ： Control group, Toxic group, and Intervention groups. The 3- （4, 5-dimethyhhiazol-2-yl） -2, 5-dipheny-ltetrazolium bromide （MTF） assay and Trypan Blue staining were applied to detect the living cells percentage. Results Four methyl azo thiazole blue （MTF） assay showed the living cells percentage of the Intervention groups arised from （ 48.3±2. 0 ） % to （ 69.4±3.8 ） % , while the living cells percentage of intervention group with 80 mmol/L calcium dobesilate was obviously higher than that of the toxic group. Conclusions Nor cantharidin can effectively inhibit the proliferation of VEC and lower the living cells percentage of the cells. While the calcium dobesi- late with a concentration of 80 mmol/L shows an antagonism of the toxicity of Nor cantharidin as well as an effect of protecting the VEC.