脂联素水平及脂联素基因多态性与糖尿病视网膜病变的关系

目的研究血清脂联素（APN）水平及脂联素基因单核苷酸多态性（SNP）45T→G与2型糖尿病（T2DM）视网膜病变（DR）之问的关系。方法运用聚合酶链反应-限制性片段长度多态性方法，对76例T2DM患者无DR（NDR）组27例、非增殖型DR（NPDR）组28例、增殖型DR（PDR）组21例]，和35例正常对照（Nc）者的APN基因SNP45多态性位点进行基因分型；用放射免疫法检测空腹血清APN浓度；比较各组基因型和等位基因频率，并分析各指标间的相关性。结果（1）T2DM组血清APN水平明显低于NC组（P＜0.01）；PDR组APN浓度明显低于NDR和NPDR组，差异有统计学意义（P均＜0.01）；（2）血清APN浓度与年龄、收缩压、空腹血糖、空腹胰岛素、HbA1C、TG、LDL-C、HO—MA—IR呈显著负相关；（3）SNP45多态性位点的基因型和等位基因频率在NDR、NPDR、PDR组和NC组三间无统计学差异（P〉O．05），三种基因型的血清APN水平无统计学差异（P＞0.05）。结论T2DM患者血清APN水平降低，APN在DR的发病机制中发挥作用且与DR的严重度相关；而APN基因SNP45多态性位点与青岛地区汉族人群中DR无明显相关性。

Association of adiponectin gene polymorphism and serum adiponectin level with diabetic retinopathy

Objective To investigate the association of single nucleotide polymorphism （SNP） of 45 T→G of adiponectin gene and serum adiponectin level with type 2 diabetic retinopathy. Methods PCR- RFLP method was used to determine the distributions of allele and genotype frequencies of SNP45 T→G polymorphism in 76 type 2 diabetic patients and 35 normal control subjects. The patients were stratified into three subgroups ： without retinopathy （NDR, n = 27）, non-proliferative diabetic retinopathy （NPDR, n=28） and proliferative diabetic retinopathy （PDR, n = 21）. Serum adiponectin concentration was determined by RIA,and compared with genotype and allele frequencies. Results （1） The level of serum adiponectin was significantly decreased in diabetes group compared with control group （P〈0.001）. Serum adiponectin concentration was significantly lower in PDR group than in NDR and NPDR groups. （2） Serum adiponectin was correlated negatively with age, systolic blood pressure, fasting plasma glucose, fasting insulin, glycated haemoglobin, insulin resistance index, triglyceride and LDL cholesterol. （3） The distributions of genotype and allele frequencies of T→G polymorphism of SNP 45 were not different among three subgroups and control group （P〉0.05）, and serum adiponectin levels in three genotypes were not different. Conclusions Adiponectin concentration is lower in patients with type 2 diabetes and the T→G oolymorphism of SNP 45 in adiponectin gene is not associated with diabetic retinopathy in Qingdao Han population.