| Rho相关卷曲螺旋形成蛋白激酶1促进肝癌细胞的转移 |
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| 引用本文： | 倪庆锋,孔连宝,刘 静,蔡卫华.Rho相关卷曲螺旋形成蛋白激酶1促进肝癌细胞的转移[J].南京医科大学学报,2013(10):1377-1382. |
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| 作者姓名： | 倪庆锋 孔连宝 刘 静 蔡卫华 |
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| 作者单位： | 南通大学附属南通第三医院普外科,江苏 南通 226000;南京医科大学第一附属医院肝脏外科,江苏 南京 210029;南通大学附属南通第三医院普外科,江苏 南通 226000;南通大学附属南通第三医院普外科,江苏 南通 226000 |
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| 基金项目： | 国家自然科学基金项目(81272229) |
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| 摘 要： | 目的:探讨Rho相关卷曲螺旋形成蛋白激酶1(ROCK1)在肝癌中的表达及在肝癌侵袭中的作用?方法:荧光定量PCR(real-time PCR)检测肝癌及癌旁组织中ROCK1的mRNA表达水平,同时检测肝癌细胞系及正常人肝细胞中ROCK1mRNA表达水平,免疫组织化学染色(IHC)法检测肝癌及癌旁组织中ROCK1蛋白的表达量,并通过在肝癌细胞株中过表达及干扰ROCK1分析ROCK1蛋白对肝癌细胞转移的作用?结果:60对样本中有42对ROCK1的mRNA表达水平在肝癌组织中高于癌旁组织,免疫组织化学染色提示肝癌组织中ROCK1蛋白表达量高于癌旁组织?在体外肝癌细胞的功能实验中,过表达ROCK1能促进HepG2细胞的侵袭和转移,而干扰ROCK1的表达能减少SMMC-7721细胞的侵袭和转移?结论:ROCK1在肝癌组织中高表达,过表达ROCK1能促进肿瘤的侵袭及转移,ROCK1可能成为临床治疗中抑制肿瘤侵袭和转移的靶点?
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| 关 键 词： | ROCK1 肝细胞性肝癌 肿瘤侵袭 |
| 收稿时间： | 6/3/2013 12:00:00 AM |
| 修稿时间： | 2013/9/10 0:00:00 |
Ectopic overexpression of ROCK1 promotes cellular invasion of hepatocellular carcinoma |
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| Ni Qingfeng,Kong Lianbao,Liu Jing and Cai Weihua.Ectopic overexpression of ROCK1 promotes cellular invasion of hepatocellular carcinoma[J].Acta Universitatis Medicinalis Nanjing,2013(10):1377-1382. |
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| Authors: | Ni Qingfeng Kong Lianbao Liu Jing and Cai Weihua |
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| Institution: | Department of General Surgery,the Third Affiliated Hospital of Nantong University,Nantong 226000;Department of Hepatic Surgery,the First Affiliated Hospital of NJMU,Nanjing 210029,China;Department of General Surgery,the Third Affiliated Hospital of Nantong University,Nantong 226000;Department of General Surgery,the Third Affiliated Hospital of Nantong University,Nantong 226000 |
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| Abstract: | Objective:To detect the expression and explored the role of ROCK1 in hepatocellular carcinoma (HCC). Methods:The expression of ROCK1 mRNA in HCC samples and cell lines was detected by real-time PCR,while the expression of ROCK1 protein in HCC tissues and adjacent non-cancer tissues was assessed by immunohistochemistry. In addition,invasion of HCC cells was observed after overexpressing or silencing ROCK1. Results:In the total of 60 paired HCC specimens,compared with the adjacent non-cancer tissues,the expression of ROCK1 mRNA was up-regulated in 42 cases(P < 0.05),and the expression of ROCK1 protein in HCC tissues was higher than that in adjacent non-cancer tissues. Overexpression of ROCK1 enhanced HCC-derived HepG2 cellular invasion in vitro. In contrast,ROCK1 knockdown via RNAi markedly suppressed these phenotypes in SMMC-7721. Conclusion:This study indicates ROCK1 is frequently overexpressed in hepatocellular carcinoma,and contributes to tumor cell invasion. ROCK1 may be a novel therapeutic target for prevention and treatment of invasion in HCC. |
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| Keywords: | ROCK1 hepatocellular carcinoma tumor invasion |
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