新诊断2型糖尿病患者外周血单个核细胞中JNK活性变化及其影响因素

目的了解新诊断2型糖尿病外周血单个核细胞中氨基末端激酶（JNK）（Thr^183／Tyr^185）活性变化及其影响因素。方法招募正常糖耐量健康志愿者60名（NGT组），新诊断2型糖尿病患者60例（NT2DM组）。应用western blot法检测外周血单个核细胞中JNK（Thr^183／Tyr^185）磷酸化水平。结果NT2DM组JNK（Thr^183／Tyr^185）磷酸化水平显著高于NGT组，差异有统计学意义（P〈0．01）。相关性分析显示，JNK（Thr^183／Tyr^185）磷酸化水平与体重指数（BMI）、空腹血糖（FBG）、低密度脂蛋白胆固醇（LDL—C）呈正相关（P〈0．05），在剔除年龄、性别等相关影响因素之后，空腹血糖仍与JNK（Thr^183／Tyr^185）磷酸化水平独立相关。结论JNK通路过度激活可能增加了患糖尿病的风险。

JNK activity in peripheral blood mononuclear cells of newly diagnosed type 2 diabetic patients and its influencing factors

Objective To explore the change of JNK （Thr^183/Tyr^185） activity in the peripheral blood mono- nuclear cells in the newly diagnosed type 2 diabetes patients. Methods A total of 60 healthy volunteers with normal glucose tolerance （NGT group）, 60 newly diagnosed type 2 diabetes patients （NT2DM group） were recruited in our study. JNK （Thr^183/Tyr^185 ） phosphorylation levels were deleeted in the peripheral blood mononuclear cells by western blot method. Results JNK （ Thr^183/Tyr^185 ） phosphorylation levels in NT2DM group were significantly higher than that in NGT group （P 〈 0. 01 ）. The correlation analysis showed that JNK （Thr^183/Tyr^185） phosphorylation levels were positively associated with body mass index, fasting blood glucose and low-density cholesterol （ P 〈 0. 05 ）. Under the situation of excluding the related factors such age, gender and so on, JNK （ Thr^183/Tyr^185 ） phosphorylation levels were still independently associated with fasting blood glucose （ P 〈 0. 05）. Conclusion JNK pathway over-activation may increase the risk of diabetes.