1,2-二氯乙烷致脑水肿过程中自由基的作用研究

目的：探索自由基在1，2－二氯乙烷（1，2－DCE）致脑水肿过程中所起的作用。方法：在复制DCE脑水肿模型的基础上，检测动物血清和脑组织中丙二醛（MDA），谷胱甘肽（GSH）含量及超氧化物歧化酶（SOD）活力的变化。结果：随着染毒学的增加，血清中MDA的变化由低到高，除低浓度组与中浓度组比差异无显著性外，其余各组之间相比差异都具有显著性（P＜0．05），而SOD则先增加后降低，对照组的SOD活力在染毒前后变化不明显；低和中浓度组SOD活力在染毒后增加（P＜0．05），高浓度组SOD活力染毒后大幅降低（P＜0．05），GSH随着浓度的增加，含量逐渐降低，降低浓度组与对照组差异无显著性外（P＞0．05），其余各组之间相比差异有显著性（P＜0．05），脑组织中随着染毒浓度的增加，MDA逐渐增加，除低浓度组与对照组差异无显著性外（P＞0．05），高、中浓度组与对照组比差异有显著性（P＜0．05），SOD值逐渐减小，高，中浓度组与对照组相比，差异有显著性（P＜0．05），GSH先升后降、高、低浓度组与对照组相比差异具有显著性（P＜0．05），结论：1，2－DCE致脑水肿过程中，自由基所起的作用不容忽视。

Research on the effect of free radical in the process of cerebral edema induced by 1,2-dichloroethane

ObjectiveTo study the effects of free radica l in the process of cerebral edema induced by 1,2 dichloroethane (DCE). MethodsOn the basis of duplicating the animal model of cerebra l edema induced by DCE, MDA, GSH contents and SOD activity in serum and brain t issue were examined.Results With the increase of DCE concentrations, MDA in brain tissue and serum also increased and a significant difference was observed between the higher concentration group and the control g roup(P<0.05). SOD decreased gradually while the concentrations incr eased(P<0.05 in the high/medium concentration groups). GSH content in se rum decreased when concentration getting higher(P<0.05 in the h igh/me dium concentration groups). GSH content in the brain increased (P<0.05 in the low and medium concentration groups) and then decreased (P<0.05in the high concentration group).ConclusionIt suggested that f ree radical might have definite effects in the process of cerebral edema induced by DCE.