挥发性有机物对永生化支气管上皮细胞的急性毒性

目的评估家装材料中典型挥发性有机物(VOCs)混合物体外条件下对永生化支气管上皮细胞(BEAS-2B)所致的急性毒性。方法选取装修材料中常见的12种挥发性有机物及其混合物作为研究对象,以液体染毒方式处理细胞,检测染毒后细胞的LC50、克隆存活、活性氧(ROS)产生和胞外乳酸脱氢酶(LDH)的变化,确定VOCs混合物的急性毒性。结果各VOCs作用于细胞后,其LC50值由0.70 g/L到22.80 g/L不等,而VOCs混合物细胞的LC50和克隆存活的LC50分别为0.82 g/L和0.30 g/L,毒性明显升高,展示出独特的化学性质和毒性特质,表明VOCs混合物的毒性效应是12种成分毒性效应的联合作用(协同、加强、超相加)的结果。同时VOCs混合物还诱导BEAS-2B细胞产生大量活性氧.OH和O2.-,使胞外LDH升高,且各效应与毒物作用剂量成正相关关系。结论VOCs混合物细胞毒性明显升高,表现为各成分的联合作用。它可诱导BEAS-2B细胞产生活性氧(ROS),引起细胞膜受损而死亡。且VOC混合物的急性毒性效应在高剂量时呈现细胞致死效应,较低剂量时呈现一种细胞转化效应。

Volatile Organic Compounds (VOCs)-Induced Acute Cytotoxicity to Human Bronchial Epithelial Cell Line BEAS-2B

Objective The aim of this study was to evaluate the acute cytotoxicity of typical VOCs mixture from decorative & building materials to human bronchial epithelial cell line BEAS-2B in vitro.Methods Individual and the mixture of 12 often-detected VOCs from decorative materials were tested for their acute cytotoxicity to human bronchial epithelial cell line BEAS-2B.BEAS-2B is one of the adenovirus-12/SV40 hybrid virus immortalized human bronchial epithelial cell lines.Cytotoxicity was measured by detection of survival LC_(50),reactive oxygen species(ROS),and lactate dehydrogenase(LDH).Results The LC_(50) of individual varied greatly from 0.70 g/L to 22.8 g/L.But VOCs mixture showed a special cytotoxic profile-its LC_(50) was 0.82 g/L and its clone survival LC_(50) was 0.30 g/L.These results indicate that the VOCs mixture is superior than individual components,and this strong toxicity may be from addictive effects of those 12 individual components.VOCs also induced ROS production and LDH leakage from BEAS-2B cells.The VOCs mixture increased more ROS production and LDH leakage than individual VOCs.Conclusion The VOCs mixture caused stronger cytotoxicity than individual components.VOCs induced cell death of BEAS-2B by increasing ROS production and damaging cell membranes.Whereas lower dose of VOCs mixture had a transforming effect,the high dose of the mixture caused BEAS-2B cell death.