| 谷胱甘肽与顺铂合用对肝癌细胞HepG_2增殖及凋亡的影响 |
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| 引用本文： | 徐艳岩,安郁宽,李波清,李京敏. 谷胱甘肽与顺铂合用对肝癌细胞HepG_2增殖及凋亡的影响[J]. 中国老年学杂志, 2010, 30(4) |
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| 作者姓名： | 徐艳岩 安郁宽 李波清 李京敏 |
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| 作者单位： | 滨州医学院,山东,滨州,256603 |
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| 基金项目： | 滨州医学院科技计划课题 |
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| 摘 要： | 目的 探讨谷胱甘肽 (GSH) 与顺铂 (CDDP) 联合应用对人肝癌细胞HepG_2细胞增殖及凋亡的影响.方法 应用酸性磷酸酶法 (APA) 检测细胞生长抑制率;用倒置显微镜观察经CDDP、GSH处理后的细胞形态学改变;采用流式细胞术研究GSH与CDDP合用对细胞周期的影响;AnnexinV-FITC/PI双染检测细胞凋亡率;罗丹明123染色检测线粒体膜电位 (Δψm) 变化.结果 GSH与CDDP联合应用可以明显抑制HepG_2细胞增殖,癌细胞出现凋亡形态变化,其作用明显高于GSH组与对照组细胞,且不低于CDDP单独给药组;GSH与CDDP联合应用引起Δψm更明显地下降.结论 GSH与CDDP联合用药不降低CDDP对肝癌细胞的作用,其诱导肝癌细胞HepG_2凋亡的作用可能是通过线粒体通路实现的.
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| 关 键 词： | 谷胱甘肽 顺铂 肝癌细胞 凋亡 细胞周期 |
Effect of glutathione combined with cisplatin on the proliferation and apoptosis of HepG_2 cells |
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| XU Yan-Yan,An Yu-Kuan,LI Bo-Qing,et al.. Effect of glutathione combined with cisplatin on the proliferation and apoptosis of HepG_2 cells[J]. Chinese Journal of Gerontology, 2010, 30(4) |
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| Authors: | XU Yan-Yan An Yu-Kuan LI Bo-Qing et al. |
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| Affiliation: | XU Yan-Yan,An Yu-Kuan,LI Bo-Qing,et al. Binzhou Medical College,Binzhou256603,Sh,ong,China |
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| Abstract: | Objective To explore the effect of glutathione (GSH) combined with cisplatin (CDDP) on the proliferation and apoptosis of HepG2 cells. Methods Acid phosphatase assay (APA) was used to determine the inhibition rate of cells. Inverted microscope was used to observe the morphological changes of cells after CDDP and GSH treatment. Flow cytometry was used to study the effect of GSH combined with CDDP on the cell cycle. AnnexinV-FITC/PI was carried out to evaluate the apoptosis. The change of mitochondrial membra... |
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| Keywords: | Glutathione Cisplatin Hepatoma Apoptosis Cell cycle |
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