翼状胬肉标本中RhoC和MMP-9的表达及相关性研究

目的研究RhoC蛋白和基质金属蛋白酶-9(MMP-9)在翼状胬肉中的表达及二者的相关性,探讨利用RhoC及MMP-9作为标记物评估翼状胬肉生物学特性的可行性。方法应用辣根过氧化物酶标记的链霉卵白素免疫组织化学方法(S-P法)对45例原发性翼状胬肉和16例正常球结膜组织中的RhoC及MMP-9蛋白进行检测。结果 RhoC及MMP-9蛋白在翼状胬肉组织中的表达均显著高于正常结膜组(P均0.05)。RhoC及MMP-9蛋白表达之间的相关系数r=0.562,有显著性差异(P0.05)。结论检测RhoC及MMP-9在翼状胬肉中的表达,可有效判断患者翼状胬肉的发病及复发情况,以便于早期采取相关治疗措施,减轻患者痛苦及经济负担。

Study on the expression and correlation of RhoC and MMP-9 in pterygium specimens

Objce tive It is to observe the expression and correlation of RhoC protein and matrix metalloproteinases 9 （MMP 9） in pterygium, and explore the feasibility by using RhoC and MMP -9 as a marker to evaluate pterygium biological characteristics .Methods The expression of RhoC and MMP -9 in 45 cases of primary pterygium and 16 cases of normal con-junctival tissue were detected by horseradish peroxidase labeled chain mildew avidin method of immunohistochemistry （ S-P method）.Results RhoC protein and MMP -9 protein expression in the tissue of pterygium were higher than that in normal conjunctival group, there was significant difference （P〈0.05）.The correlation coefficient between RhoC and MMP -9 pro-tein r=0.562, there is significant difference （P〈0.05）.Conclusion The expression of RhoC and MMP -9 in pterygium can effectively determine the incidence and recurrence of pterygium , so as to take relevant measures in the early treatment , to alle-viate the suffering and economic burden of patients .