| 人解痉多肽原核表达载体的构建及诱导表达 |
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| 引用本文： | 孙勇,孙曙光,吴炜,张勇,吕尚军,王林,彭曦,汪仕良.人解痉多肽原核表达载体的构建及诱导表达[J].南京医科大学学报,2008,28(12):1537-1540. |
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| 作者姓名： | 孙勇 孙曙光 吴炜 张勇 吕尚军 王林 彭曦 汪仕良 |
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| 作者单位： | 中国人民解放军第97医院烧伤整形科，第三军医大学西南医院全军烧伤研究所，创伤、烧伤与复合伤国家重点实验室 |
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| 基金项目： | 国家自然科学基金资助项目
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| 摘 要： | 目的:构建人解痉多肽(hSP)原核表达载体,表达重组hSP,为功能研究奠定基础。方法:通过RT-PCR获得hSPcDNA片段,将目的基因插入原核表达载体pET32a,得到重组载体pET32a-hSP。IPTG诱导表达后行SDS-PAGE分析及WesternBlot检测。结果:经测序及PCR证实,hSPcDNA准确插入原核表达载体pET32a中,诱导表达后SDS-PAGE分析证明hSP的分子量约为32kD,Western-blot分析表明,表达蛋白具有良好的抗原性和特异性。结论:上述结果表明成功构建出原核表达载体pET32a-hSP,获得重组hSP,并为深入研究hSP奠定了基础。
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| 关 键 词： | 人解痉多肽 大肠杆菌 诱导表达 |
| 收稿时间： | 2008/6/20 0:00:00 |
Construction of human spasmolytic polypeptide expression vector and inductively expression in E.coli |
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| Institution: | Department of Burn Surgery,Plastic Surgery Center,the 97th Hospital of PLA,Xuzhou 221004;Department of Burn Surgery,Plastic Surgery Center,the 97th Hospital of PLA,Xuzhou 221004;Institute of Burn Research,Southwest Hospital,State Key Laboratory of Trauma,Burns and Combined Injury,the Third Military Medical University,Chongqing 400038,China;Institute of Burn Research,Southwest Hospital,State Key Laboratory of Trauma,Burns and Combined Injury,the Third Military Medical University,Chongqing 400038,China;Institute of Burn Research,Southwest Hospital,State Key Laboratory of Trauma,Burns and Combined Injury,the Third Military Medical University,Chongqing 400038,China;Institute of Burn Research,Southwest Hospital,State Key Laboratory of Trauma,Burns and Combined Injury,the Third Military Medical University,Chongqing 400038,China;Institute of Burn Research,Southwest Hospital,State Key Laboratory of Trauma,Burns and Combined Injury,the Third Military Medical University,Chongqing 400038,China;Institute of Burn Research,Southwest Hospital,State Key Laboratory of Trauma,Burns and Combined Injury,the Third Military Medical University,Chongqing 400038,China |
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| Abstract: | |
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| Keywords: | human spasmolytic polypeptide E coli inductive expression |
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