Manipulation of the growth rate of human breast cancer cells by antiandrogen followed by chemotherapy |
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| Authors: | A Alama L Miglietta R E Favoni F Barbieri F Ravera L Fazzuoli G Nicolo A Nicolin |
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| Affiliation: | Department of Pharmacology, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy. |
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| Abstract: | The effects induced by the antiandrogen Cyproterone Acetate (CPA) on the proliferation of EVSA-T human breast cancer cells endowed with androgen receptors were studied. Kinetic analyses were carried out by two autoradiographic techniques measuring the percentage of cells in S-phase and the growth fraction (GF). The exposure of the cultures to CPA for 24 h caused a marked inhibition on S-phase cells without significantly affecting the CF. The accumulation of cells in G1-phase, confirmed by cytometric analysis, was rescued to the S compartment by replacing the culture medium 24 h after CPA administration. Exposure of EVSA-T to Doxorubicin or Methotrexate after CPA and medium change at the time of maximal proliferative recruitment, led to an enhancement of cytotoxicity as demonstrated by colony survival assay. |
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