| 大鼠下丘脑CCK与OT神经元的免疫组化检测 |
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| 引用本文： | 倪宏,王守彪,唐明. 大鼠下丘脑CCK与OT神经元的免疫组化检测[J]. 青岛大学医学院学报, 2000, 36(4): 261-262 |
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| 作者姓名： | 倪宏 王守彪 唐明 |
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| 作者单位： | 1. 青岛大学医学院,青岛,266021,生理学教研室
2. 青岛大学医学院,青岛,266021,解剖学教研室 |
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| 基金项目： | 山东省卫生厅科研基金资助项目(鲁卫科计字[1994]20号) |
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| 摘 要： | （１）目的 探讨缩胆囊素（ＣＣＫ）和缩宫素（ＯＴ）神经元在下丘脑的共存现象。（２）方法 采用相邻切片免疫组化方法，检测ＣＣＫ和ＯＴ样免疫活性物质（ＣＣＫ－ＩＲ，ＯＴ－ＩＲ）的分布。（３）结果 ＣＣＫ－ＩＲ广泛分布在下丘脑的视上核，室周核，视交叉上核，腹内侧核和弓状核等区域，由中等大小卵圆形细胞构成，室旁核末检测到ＣＣＫ－ＩＲ；ＯＴ－ＩＲ仅局限于室旁核和视上核，由大细胞构成。（４）结论 ＣＣＫ和ＯＴ
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| 关 键 词： | 下丘脑 缩胆囊素 缩宫素 免疫组织化学 大鼠 |
| 文章编号： | 1001-4047(2000)04-0261-02 |
| 修稿时间： | 2000-01-10 |
ADJACENT SECTIONS IMMUNOHISTOCHEMICAL STUDY ON THE COEXISTENCE OF CHOLECYSTOKININ WITH OXYTOCIN IN RAT HYPOTHALAMUS |
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| NI Hong,WANG Shoubiao,TANG Ming. ADJACENT SECTIONS IMMUNOHISTOCHEMICAL STUDY ON THE COEXISTENCE OF CHOLECYSTOKININ WITH OXYTOCIN IN RAT HYPOTHALAMUS[J]. Acta Academiae Medicinae Qingdao Universitatis, 2000, 36(4): 261-262 |
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| Authors: | NI Hong WANG Shoubiao TANG Ming |
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| Abstract: | Objective To study the distribution of CCK immunoreactive(CCK IR) and OT immunoreactive(OT IR) cells in the hypothalamus of rats. Methods Using the mirror sectioning immunohistochemical technique, the coexistence of CCK IR and OT IR cells in hypothalamus was examined with ABC immunostaining method on adjacent mirror thin sections. Results CCK IR cells distributed mainly in supraoptic nucleus(SON), suprachiasmatic nucleus(SCN), periventricular hypothalamic nucleus (PVN), arcuate hypothalamic nucleus(ARCN)and ventromedial hypothalamic nucleus(VMH); OT IR cells distributed only in SON and PVN. Conclusion This study reveals that CCK IR cells and OT IR cells co exist in SON, but they do not present in the same cells,for CCK IR cells are parvocellular cells,while OT IR cells are magnocellular cells. [ |
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| Keywords: | hypothalamus cholecystokinin oxytocin immunohistochemistry rats |
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