氯化锂阻断大鼠海马注射溶血磷脂酸诱导的神经元Tau蛋白磷酸化

目的:探讨氯化锂(LiCl)对溶血磷脂酸(LPA)在大鼠体内引起海马神经细胞tau蛋白磷酸化水平的影响。方法:将54只SD大鼠分为LPA实验组9只,LiCl+LPA实验组27只(低、中、高量LiCl组各9只),实验对照组9只,对照组9只,采用腹腔注射LiCl,脑立体定位仪技术将LPA、PBS缓冲液注射入大鼠海马,各实验组大鼠分别于注射LPA后12,24,48 h后处死,对照组大鼠未做任何处理直接处死,采用免疫组化方法测定海马锥体细胞中ser202位点磷酸化tau蛋白(PS202-tau)的表达。结果:LiCl+LPA实验组Tau蛋白磷酸化的水平均低于相同时间点处死的LPA实验组(P<0.05),且LiCl浓度越高,Tau蛋白磷酸化的水平越低。结论:LiCl能抑制LPA引起的大鼠海马神经细胞的Tau蛋白磷酸化。

Effect of Lithium Chloride on Hyperphosphorylation of Tau Protein Induced by Lysophosphatidic Acid in Rat's Hippocampual Neurons

Objective: To observe the effect of lithium chloride(LiCl) on the hyperphosphorylation of tau induced by Lysophosphatidic acid(LPA) in rat's hippocampual neurons.Methods: Fifty-four adult SD rats were assigned to six groups randomly as following: LPA injection group(n=9),three different dose of LiCl(low,moderate,high) LPA injection groups(n=9 in each group),vehiculum injection group(n=9) and normal control group(n=9).Stereotaxic microinjection of LPA or vehiculum into hippocampus were performed in LPA injection group,vehiculum injection group and normal control group,while different doses of LiCl were injected peritoneally and 24 hours after the Stereotaxic microinjection of LPA were performed in LiCl LPA groups.At interval time of 12,24 and 48 h after LPA injection,the expression of PS202-tau was observed by immunohistochemical assay.Results: Compared with LPA injection group,the expression of PS202-tau in LiCl LPA groups was lower in rats which executed at same interval time,and the higher dose of LiCl,the lower the expression of PS202-tau.Conclusion: In vivo,LiCl injection could inhibit the hyperphosphorylation of tau induced by LPA in rat's hippocampual neurons.