汉防己甲素治疗急性脊髓损伤的实验研究

目的 探讨汉防己甲素(Tetrandrine，Tet)对急性脊髓损伤(Acute spinal cord injuries，ASCI)的保护作用。方法 74只Wistar大鼠随机分为4组，用改良Allen’s打击法制备大鼠截瘫模型，造模前各组分别投给相应的药物，伤后1h、4h取损伤区脊髓组织进行形态学观察和生化指标测定。结果 汉防己甲素能明显降低组织钙总量和MDA含量，防止镁总量和SOD含量的下降，稳定6-Keto-PGFla／TXB2的比值，减轻组织病损。结论 Tet对ASCI有保护作用，其作用机制为①扩张微血管，改善微循环，逆转ASCI后早期缺血倾向；②抗氧自由基，减轻组织过氧化损伤，稳定生物膜性结构；③减少ASCI后钙内流，从而阻断继发性损伤的链式反应。

An experimental study of Tetrandrine (Tet) for the treatment of acute spinal cord injury in rats

Objective To study the protective effect and mechanism of tetrandrine on acute spinal cord injury (ASCI) in rats Methods Seventy-four Wistar rats both male and female were divided into four groups.The spinal cord injury models in rats were produced by 100 g.cm force resulted in paralegia by modified Allen's technique.Tet was administered before ASCI.One hour or four hours after the traumatic injury,samples of spinals cord were taken out for biochemical assay and examination of the pathological changes.Results Tet decreased significantly calcium concentration and content of MDA,prevented megnesium concentration and content of SOD from decreasing,stabilized the ratio of 6-Keto-PGF 1a/TXB 2 and lessoned the pathologic injuries of spinal cord.The effects were similar to that of MP.Conclusion The results of present study suggests that tetrandrine posseses a protective effects on contused spinal cord in rats,in which the mechanisms include the ability of dilating micro-vasculature,ameliorating local microcirculation of injuries spinal cord,overcoming early posttraumatic ischemia of the cord,preventing the production of relative oxyen metablites,attenuating the injury of peroxidation,stabilizing the membranaceous structure and reducing the movement of extracellular calcium to intracellular spaces,thereby restraining a cascade of secondary injury.