蛇床子对运动训练大鼠睾酮含量、物质代谢 及抗运动疲劳能力的影响

目的: 研究蛇床子对运动训练大鼠睾酮含量、物质代谢及抗运动疲劳能力的影响。方法: 以大强度耐力训练大鼠为模型,80只42 d龄雄性Wistar大鼠为对象,随机分为5组:静止对照组(C组)、运动对照组(T组)、运动+低剂量蛇床子组(TML组)、运动+中剂量蛇床子组(TMM组),运动+高剂量蛇床子组(TMH组)每组15只(剔除不符合实验要求的大鼠)。每天灌胃(ig)给药1次,蛇床子组剂量分别为0.75,1.5,4.5 g·kg^-1,ig体积为5 mL·kg^-1,静止对照组、运动对照组ig等量生理盐水。42 d力竭游泳训练后,测定体重、力竭游泳时间及血睾酮等生化指标。结果: 蛇床子各剂量组体重大于运动组(P<0.05),力竭游泳时间长于运动组(P<0.01);血清睾酮高于运动对照组 (P<0.01);血清皮质酮水平各组间均无显著差异,各组间血清睾酮与皮质酮比值变化与睾酮变化较为一致;肝糖原(P<0.05)、肌糖原(P<0.01)高于运动对照组;促卵泡激素无明显影响;血尿素低于运动对照组(P<0.05),血红蛋白高于运动对照组 (P<0.05)。结论: 蛇床子可以减轻血睾酮受高强度运动量的影响,并维持在正常生理水平;可以促进蛋白质合成,抑制氨基酸和蛋白质分解,提高运动训练大鼠血红蛋白含量和糖原的储备。

Effect of Fructus cnidii on Testosterone Content, Substance Metabolism and Exercise Capacity in Rats Receiving Exercise Training

Objective: To study the effects of Fructus cnidii on the content of testosterone, substance metabolism and anti-fatigue ability of rats. Method: By using the model of high-intensity endurance training, 80 male Wistar rats were divided into 5 groups, 15 in each group (the rats which did not meet the requirement were removed):control group (C group), motion control group (T group), exercise+ig low-dose F. cnidii group (TML group), exercise+ig middle-dose F. cnidii group (TMM group), and exercise+ig high-dose F. cnidii group (TMH group). Gavage was performed using professional device once a day. The rats in F. cnidii groups were treated with 0.75,1.5, 4.5 g·kg^-1. The rats in C and T groups were given saline of same volume. After 42 days of exhaustive swimming training, body weight, swimming time and serum testosterone and other biochemical markers were measured. Result: The body weight of the rats in F. cnidii groups increased compared with T group (P<0.05), and the swimming time was longer than T group (P<0.01), serum corticosterone was lower than the T group (P<0.05), changes in the ratio of serum testosterone/corticosterone were more consistent with testosterone changes among the groups. Liver glycogen (P<0.05), muscle glycogen(P<0.01) and hemoglobin(P<0.05) in F. cnidii group were higher than in T group, but the serum urea nitrogen was lower than the T group (P<0.05). Conclusion: F. cnidii can alleviate the impact of high-intensity exercise on serum testosterone, and maintain it at normal physiological levels; it can also promote protein synthesis, inhibit degradation of amino acid and protein, and increase hemoglobin and glycogen reserves in rats exercise training.