加味温胆汤抗大鼠营养性肥胖的机制探讨

目的: 从血清总超氧化物歧化酶(T-SOD)、一氧化氮(NO)、丙二醛(MDA)含量和肝、脂肪组织病理学改变角度探讨加味温胆汤抗大鼠营养性肥胖的机制。方法: 采用"髙脂乳剂+普通饲料"双重喂养法复制大鼠营养性肥胖模型。雄性断乳SD大鼠30只,按体重均匀分为空白对照组、模型对照组和加味温胆汤组,每组10只。各组动物每天均喂饲普通饲料,除空白对照组外,其余组动物每天上午灌胃给予自制高脂乳剂20 mL·kg^-1造模,连续4周。从第5周开始,于每天下午灌胃给予实验组加味温胆汤(7.74 g·kg^-1),空白对照组与模型对照组给同体积水,连续4周。末次给药后,禁食不禁水12 h,取血清检测T-SOD,NO,MDA水平;分离肝、脂肪组织,10%甲醛溶液固定,常规制片,HE染色,光镜下观察肝、脂肪细胞的病变。结果: 加味温胆汤组大鼠血清NO含量明显低于模型组(P<0.01);大鼠肝细胞界限清楚,排列整齐,脂变肝细胞散在;脂肪细胞数量增多,体积减小。结论: 加味温胆汤抗大鼠营养性肥胖的机制可能与降低血清NO水平、对抗肝细胞脂肪变性、缩小脂肪细胞体积等有关。

On the Mechanism of Jiawei Wendan Decoction Fighting Nutritional Obesity of Rats

Objective: To study the mechanism of Jiawei Wendan decoction fighting the nutritional obesity of rats by the contents of serum levels of total superoxides, such as dismutase (T-SOD), nitric oxide(NO), malondialdehyde(MDA), and the aspect of the pathological changes of liver and adipose tissue. Method: Adopting the double feeding method of high fat emulsion and general feed to copy the model of rats with nutritional obesity. Thirty male SD rats of ablactation were equally divided into the blank control group, model control group and the Jiawei Wendan decoction group. Animals in each group were fed with normal diet every day. Except the control group, animals in the other two groups were treated with intragastric administration of self-made high fat emulsion(20 mL·kg^-1) every morning for 4 weeks. From the fifth week to the eighth week, Jiawei Wendan decoction 7.74 g·kg^-1 was given every afternoon orally, and animals in the control group and model control group were given with the same volume of water. After the last administration, there was a fasting of 12 h, but water was available. The serum level of T-SOD, NO, MDA were tested; separating liver and adipose tissue, hematoxylin and eosin (HE) staining, and the structure and pathological change of liver, adipose cells were observed under optical microscope. Result: The serum NO content in the experimental scattered Jiawei Wendan decoction group was significantly lower than those in the model group (P<0.01); the rats' liver cell boundaries are clear and neat;with scattered fatty degeneration of liver cells; the number of fat cells increased and the volume of these cells decreased. Conclusion: The mechanism of Jiawei Wendan decoction for anti-nutritional obesity in rat may be related to the decrease in the level of serum NO, fighting against the fatty degeneration of liver cells, and reducing the volume of fat cell.