BARF1基因的启动子活性及其在鼻咽癌细胞中肿瘤特异性分析研究

目的探讨BARF1基因的启动子活性及其在鼻咽癌细胞中是否具有肿瘤相对特异性。方法结合启动子预测软件结果(TRANSFAC和MATINSPECTOR)及引物设计软件(Primer premier 5.0),设计BARF1基因的启动子序列,验证其启动子活性,并比较该启动子在NP69细胞和CNE-2细胞中的的启动子活性的差异,验证其肿瘤相对特异性。结果测序和酶切结果证明所设计的BARF1基因的启动子的序列完全正确,其在CNE-2细胞中具有启动子活性,而在NP69细胞中不具备启动子活性,且该启动子在CNE-2细胞中的启动子活性远高于NP69细胞,差异有统计学意义(<0.05)。结论本研究设计的BARF1基因的启动子具有启动子活性,且该启动子具有肿瘤相对特异性。

Analysis of promoter activity of BARF1 gene and its tumor specificity in nasopharyngeal carcinoma cells

Objective To explore the promoter activity ofBARF 1 gene and its tumor specificity in nasopharyngeal carcinoma cells. Methods Combining results of promoter prediction software （ TRANSFAC and MATINSPEC- TOR） and primer design software （ Primer premier 5.0）, the sequence of BARF 1 gene promoter was designed and promoter activity was verified, and then the promoter activity was compared between NP69 cells and CNE-2 cells to explore whether this promoter had tumor relative specificity or not. Sequencing and restriction results showed that the BARF 1 gene promoter sequence of the design was entirely correct, having a promoter activity in CNE-2 cells in NP69 cells do not have a promoter activity, and the promoter of the CNE-2 promoter activity in the cells was much higher than the NP69 cell, with statistically significant （P〈0.05）. Results The designed sequence ofBARF gene promoter was proved correct by sequence testing and endonuclease digestion. BARF1 gene promoter showed promoter activity in CNE-2 cells, but there was no promoter activity detected in NP69 cells; and the promoter activity showed in CNE-2 cells was much higher than that in human nasopharyngeal epithelial cells NP69（P 〈 0.05）. Conclusions The promoter sequence of BARF1 gene has promotive activity, and also has tumor relative specificity.