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HPLC测定农田土、腐殖土西洋参皂苷含量及对比与分析
引用本文:刘哲,武晓林,张连学.HPLC测定农田土、腐殖土西洋参皂苷含量及对比与分析[J].中国实验方剂学杂志,2013,19(19):79-81.
作者姓名:刘哲  武晓林  张连学
作者单位:吉林医药学院, 吉林 吉林 132013;吉林农业科技学院, 吉林 吉林 132101;吉林农业大学, 长春 130118
基金项目:国家科技支撑计划项目(2007BA138B01)
摘    要:目的: 以西洋参皂苷为指标,比较2种土壤环境种植西洋参皂苷的含量。方法: 采用药典方法提取西洋参皂苷,用紫外分光光度计测定总皂苷含量,HPLC测定8种单体皂苷的含量。结果: 2种土壤环境种植西洋参的总皂苷含量均>6%,腐殖土西洋参单体皂苷含量高于农田土西洋参单体皂苷含量。结论: 2种土壤西洋参总皂苷含量无明显差异;各单体皂苷中腐殖土西洋参Rc,Re含量略高于农田土西洋参。

关 键 词:不同土壤环境  西洋参  总皂苷  单体皂苷
收稿时间:8/6/2012 12:00:00 AM

Determination and Contrast Analysis on Ginsenosides Contents of Panax quinquefolium Cultivated in Farmland or Humus Soils
LIU Zhe,WU Xiao-lin and ZHANG Lian-xue.Determination and Contrast Analysis on Ginsenosides Contents of Panax quinquefolium Cultivated in Farmland or Humus Soils[J].China Journal of Experimental Traditional Medical Formulae,2013,19(19):79-81.
Authors:LIU Zhe  WU Xiao-lin and ZHANG Lian-xue
Institution:Jilin Medical College, Jilin 132013, China;Jilin Agricultural Science and Technology College, Jilin 132101, China;Jilin Agricultural University, Changchun 130118, China
Abstract:Objective: To determine and analyze the ginsenosides contents of Panax quinquefolium which cultivated in farmland or humus soils. Method: Ginsenoside was extracted to determine the total content of ginsenosides with the ultraviolet spectrophotometry, and 8 kinds of monoginsenosides were measured with HPLC. Result: The content of total ginsenosides from two kinds of soils, were more than 6% and the content of monoginsenosides in humus soil was more than that in farmland soil. Conclusion: The total ginsenosides content is not different obviously; but the monoginsenoside Rc and Re in humus soil is higher.
Keywords:different soil  Panax quinquefolium  total ginsenosides  monoginsenosides
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