网织血小板检测方法的改良及临床应用

目的 改良网织血小板（RP）检测方法并探讨其临床应用价值。方法 以噻唑橙（TO）作为RNA的荧光染料，利用流式细胞仪检测30例正常人（正常对照组）和48例各种血小板减少症外周血中含有RNA的RP百分数及RP绝对值，后者包括原发性血小板减少性紫癜（1TP）、再生障碍性贫血病人（AA）及初治急性白血病病人。结果ITP病人RP百分数明显高于正常对照组（F=65．29，q=10．26，P〈0．01），而RP绝对值明显低于正常对照组（F=85．07，q=19．59，P〈0．01）；AA病人RP百分数及绝对值均低于正常对照组（q=3．47、16．41，P〈0．05、0．01）；AL组病人RP百分数与正常对照组比较无明显差异（q=0．51，P〉0．05），但其RP绝对值明显低于正常对照组（q=15．46，P〈0．01）。结论 改良了传统的网织血小板检测方法并建立了简便、稳定的检测手段，为临床各类血小板减少性疾病的诊断提供了可靠的方法。

IMPROVEMENT AND CLINICAL APPLICATION OF RETICULATED PLATELET DETECTION

ObjectiveTo improve the measurement of reticulated platelets(RPs) and to explore its clinical application. MethodsUsing thiazole orange as a fluorescent dye,RPs were measured by analyzing the RNA content in platelets in 30 normal controls and 48 various thrombocytopenia,i.e.idiopathic thrombocytopenic purpura(ITP),aplastic anemia(AA),and initially-treated acute leukemia(AL),with flow cytometry.The percentage and absolute value of RPs were calculated.ResultsCompared with the normal group,ITP patients had a significantly high percentage(F=65.29,q=10.26,P<0.01) and low absolute value of RPs(F=85.07,q=19.59,P<0.01);in AA,both the percentage and absolute value of RPs were at low levels(q=3.47,16.41;P<0.05,0.01).There was no difference of RP percentage between the AL and normal controls(q= 0.51,P>0.05),but the absolute value of RPs in AL was significantly lower than the control(q=15.46,P<0.01).ConclusionThe traditional method of reticulated platelet detection has been improved and a simple and stable one established,which provides a reliable method for diagnosis of various thrombocytopenia.