四氢生物喋呤对肾切除5／6大鼠高血压和尿蛋白的治疗作用

目的：探讨四氢生物喋呤（BH4）对肾切除5／6（NX）后大鼠血压和尿蛋白的影响。方法38只大鼠按照处理方法的不同分为五组：NX组、NX＋BH4组（腹腔注射）、NX＋L-精氨酸（LA）组、NX＋口服地尔硫卓（DILT）组和假手术组。手术24小时后开始给药，并分别在给药前和给药后的第4周和第8周测量收缩压、测定尿蛋白和肌酐清除率。在实验的第8周末观察肾脏的组织学改变。结果：与假手术组比较，NX大鼠收缩压和尿蛋白均明显升高（P〈O．01）；BH4、LA及DILT对NX所产生的高血压均有降低作用（P〈O．01）；BH4、LA和DILT均使N-X的尿蛋白明显减少（P〈0．01），但DILT的减少幅度相对较小；NX各组，血尿素和血清肌酐都升高，血浆肌酐清除率都降低，各组之间无明显差别（P〉0．05）。NX后的大鼠出现肾小球系膜扩张，而各治疗组肾小球系膜扩张的程度较轻。结论：NX大鼠，手术24小时后补充BH4，在8周的观察期内可以使血压保持正常，并有减少尿蛋白、阻止肾小球系膜扩张的作用。

Treatment of tetrahydrobiopterin supplementation on blood pressure and proteinuria in 5/6 nephrectomized rats

Objective:To explore the effect of chronic tetrahydrobiopterin(BH4)therapy on blood pressure and proteinuria in rats after 5/6 nephrectomy(NX).Methods: During an 8 week period,five groups of rats were studied: untreated 5/6 Nx rats,BH4-treated NX rats,L-arginine treated NX rats(LA),diltiazem-treated Nx rats(DILT) and sham-operated rats.Systolic blood pressure values(SBP),24h proteinuria study period.Histological changes in the kidney were evaluated at the end of the study(week 8).Results: Compared with base...