脑益康对D-半乳糖损伤的海马神经元的保护作用

目的：观察脑益康对D-半乳糖（D-gal）诱导的海马神经元损伤的作用。方法:制备脑益康含药血清。应用D-gal诱导海马神经元损伤，MTT法测定细胞活力，生化法测定细胞单胺氧化酶-B（MAO-B）、Na+-K+-ATP酶和Ca2+-ATP酶活力，RT-PCR法检测细胞bax和bcl-xl mRNA的表达。结果:脑益康含药血清可显著提高细胞活力和ATP酶活力，降低MAO-B活力，上调bcl-xl mRNA的表达，降低bax mRNA的表达。结论:脑益康可通过提高ATP酶活力，降低MAO-B活力，抑制细胞凋亡，对D-gal处理的海马神经元起到保护作用。

Protective effect of naoyikang-containing serum on cultured hippocampus neuron injury induced by D-galactose

AIM:To observe the protective effect of naoyikang-containing serum on cultured hippocampus neuron injury induced by D-galactose (D-gal). METHODS:Naoyikang-containing serum was prepared in rats administered with aqueous extract of naoyikang (6.84 g·kg-1·d-1). MTT, MAO-B and ATPase assay were used to measure the viability of hippocampus neurons. RESULTS:D-gal at concentration of 100 μmol/L caused significant decrease in the viability of hippocampus neurons 24 h after the treatment. Naoyikang-containing serum increased the viability, ATPase activity and the expression of bcl-xl mRNA, decreased the MAO-B activity and the expression of bax mRNA in D-gal injured hippocampus neurons. CONCLUSION:Naoyikang-containing serum prevents hippocampus neurons from D-gal induced cytotoxicity.