无气道高反应性的嗜酸粒细胞性支气管炎小鼠模型的建立

目的应用不同大小的变应原颗粒雾化小鼠，尝试建立具有明显的嗜酸粒细胞（EOS）气道炎症、但无气道高反应性的BALB／c小鼠模型。方法BALB／c雌性小鼠，分为3组，每组6—8只：大颗粒变应原雾化激发组（实验组）、小颗粒变应原雾化激发组（哮喘组）、生理盐水对照组（对照组）。予卵蛋白（OVA）致敏后，实验组和哮喘组第28、29、30天分别用PARI TIA喷雾器和PARI LC STAR喷雾器雾化l％ OVA 15 min激发；xff照组致敏、激发采用生理盐水。测定动物的气道反应性，观察气道炎症程度。结果哮喘组的气道反应性高于实验组和对照组，实验组与对照组的气道反应性比较差异无统计学意义（P＞0．05）。实验组肺泡灌洗液中EOS百分率高于对照组，但低于哮喘组。实验组出现支气管周围EOS等炎症细胞浸润，程度轻于哮喘组。结论采用大颗粒变应原进行雾化激发初步成功建立了具有明显的EOS气道炎症、但无气道高反应性的BALB／c小鼠模型，为进一步研究气道高反应性的发生机制奠定了基础。

Establishment of eosinophilic bronchitis mice model without airway hyperresponsiveness

Objective To explore the use of different nebulizer to establish mice model that have airway eosinophilic inflammation without airway hyperresponsiveness. Methods Female BALB/c mice were obtained and divided randomly into 3 groups： eosinophilic airway inflammation group （experimental group）, asthma group, and control group. Mice were immunized with ovalbumin （OVA）. The experiment group and asthma group were challenged with an aerosol of 1% w/v OVA using a PARI TIA and PARI LC STAR nebulizer on day 28, 29, 30, respectively. The control mice were received saline sensitization and challenge. Airway responsiveness was measured. Cell different counts in bronchial alveolus lavage fluid （BALF） were performed and a pathologist performed histopathological evaluation of the trachea and lung. Results Airway responsiveness in the experimental group was not significantly different compared with the normal saline （NS） group but was significantly different compared with the asthma group. Eosinophils in BALF were increased significantly in experimental group compared with the NS group, and significant difference was observed between experimental group and asthma group. The intensity of airway inflammation in experimental group was milder than that in the asthma model. Conclusions We established an eosinophilic bronchitis mice model without hyperresponsiveness successfully. Our model established a foundation for the further research in airway hyperresponsiveness.