细胞周期蛋白 D1和 Ki-67在喉癌组织中表达的相关性研究

目的：初步探讨细胞周期蛋白D1和Ki-67在喉鳞状细胞癌组织中表达的相关性。方法应用免疫组化链霉菌抗生物素蛋白-过氧化物酶连接法（ SP）检测Cyclin D1和Ki-67在喉鳞状细胞癌组织中的蛋白表达情况，分析两者在喉鳞状细胞癌组织中表达的相关性。结果在Ki-67阳性高表达的56例喉癌组织中，有41例出现Cyclin D1阳性高表达；在Cyclin D1阳性低度表达的51例患者中，有36例出现Ki-67阳性低度表达，经Spearman等级相关性检验：两者在喉鳞状细胞癌组织中的表达呈正相关（ rs ＝0.620， P ＜0.01）。结论 Cyclin D1和Ki-67高表达与喉鳞状细胞癌发生密切相关。

Expression and correlation of cell cycle protein-D1 and Ki-67 in laryngeal cancer tissues

Objective To investigate the expression and correlation of cell cycle protein-D1 and Ki-67 in laryngeal cancer tis-sues.Methods Immunohistochemical streptavidin-perosidase （ SP） staining method was used to detect the expression of Cyclin D 1 and Ki-67 proteins in laryngeal squamous cell carcinoma tissues and their correlation between Cyclin D 1 and Ki-67 was analyzed .Re-sults Among 56 cases of Cyclin D1 positive over-expressed laryngeal squamous cell carcinoma tissues , 41 cases of Ki-67 had positive over-expression.Among 51 cases of Ki-67 low-expressed tissues, 35 cases of Cyclin D1 had low-expression.Spearman rank correlation test showed that expressions of Cyclin D 1 and Ki-67 in laryngeal squamous cell carcinoma tissues had a significant positive correlation （ rs =0.620, P 〈0.01）.Conclusions Cyclin D1 and Ki-67 expressions in laryngeal squamous cell carcinoma have a significantly positive correlation .