| Lipofectamine^TM 2000介导pIRES2-EGFP-NT3转染小鼠耳蜗成纤维细胞的实验研究 |
| |
| 引用本文： | 陈杰,高下,麻晓峰.Lipofectamine^TM 2000介导pIRES2-EGFP-NT3转染小鼠耳蜗成纤维细胞的实验研究[J].当代医师,2014(7):891-893. |
| |
| 作者姓名： | 陈杰 高下 麻晓峰 |
| |
| 作者单位： | 南京,南京医科大学鼓楼临床医学院耳鼻210008 |
| |
| 基金项目： | 国家自然科学基金面上项目（30973302）;江苏省科教兴卫工程医学重点人才基金（RC2007010）;南京市医学重点科技发展项目（201108019） |
| |
| 摘 要： | 目的检测真核表达质粒载体pIRES2-EGFP-NT3在阳离子脂质体介导下对新生小鼠耳蜗成纤维细胞的转染情况。方法培养新生小鼠耳蜗成纤维细胞，用阳离子脂质体Lipofectamine^TM 2000介导的方法，将含有目的基因NT3的真核表达质粒载体pIRES2-EGFP．NT3对其进行转染，转染后24h，通过Confocal显微镜观察新生小鼠耳蜗成纤维细胞的转染情况。结果通过阳离子脂质体Lipofectamine^TM 2000介导，真核表达质粒载体plRES2-EGFP-NT3能有效转染新生小鼠耳蜗成纤维细胞。结论含有目的基因NT3的真核表达质粒载体plRES2-EGFP-NT3在阳离子脂质体介导下对新生小鼠耳蜗成纤维细胞的有效转染为研究NT3基因转染对正常及致聋小鼠耳蜗效应的实验奠定了基础。
|
| 关 键 词： | 阳离子 脂质体 转染 遗传载体 质粒 遗传学 耳蜗 成纤维细胞 小鼠 |
pIRES2-EGFP-NT3 transfected mouse cochlea fibroblast by lipofectamine^TM 2000 |
| |
| Authors: | Chen Jie Gao Xia Ma Xiaofeng |
| |
| Institution: | .( Department of Otolaryngology-Head and Neck Surgery, Drum Tower Hospital of Nanjing Medical University, Nanjing 210008, China) |
| |
| Abstract: | Objective To detect the transfection of pIRES2-EGFP-NT3 in mouse cochlea fibroblast by cationic liposome. Methods After pIRES2-EGFP-NT3 had been abstracted successfully, it was transfected into mouse cochlea fibroblast by lipofectamine^TM 2000. Twenty four hours later, the efficiency of the transfection was analyzed by confocal microscope. Results The plRES2-EG- FP-NT3 was effectively transfected into mouse cochlea fibroblast by cationic liposome. The transfected fibroblasts displaying green fluorescence were observed under fluorescence microscope. Conclusions The effective transfection of pIRES2-EGFP-NT3 into mouse cochlea fibroblast by lipofectamine^TM 2000 laid the basis for the following experiments, such as NT3 gene transfection in deaf or normal cochlea and so on. |
| |
| Keywords: | Cations Liposomes Transfection Genetic vectors Plasmids/genetics Cochlea Fibroblasts Mice |
| 本文献已被 维普 等数据库收录！ |
