低蛋白血症大鼠模型的建立

目的：探讨大鼠尾静脉注射阿霉素（Adriamycin，ADR）建立低蛋白血症模型的可行性。方法：SD大鼠尾静脉一次性注射阿霉素5mg／kg、7．5mg／kg及10mg／kg。观察给药后大鼠一般情况及尿量，检测尿蛋白、血清总蛋白、白蛋白、胆固醇、甘油三酯等指标，观察肾组织病理学变化，以嘌呤霉素为阳性对照。结果：阿霉素的三个剂量中，7．5mg/kg及10mg／kg组第4天开始出现尿蛋白，第15天血清总蛋白及白蛋白明显下降。两组大鼠均出现蛋白尿，血清白蛋白及总蛋白明显下降，血清胆固醇及甘油三酯升高，肾脏病理学结果符合低蛋白血症变化，但10mg／kg组大鼠死亡率高。嘌呤霉素组亦出现类似变化。结论：大鼠尾静脉注射阿霉素7．5mg／kg，15d后可建立低蛋白血症动物模型。

Establishment of adriamycin-induced hypoproteinemia model in rats

Objective:To study the feasibility of establishment of hypoproteinemia model induced by adriamycin(ADR) in rats,and provide a considerable animal model for investigating the mechanism and effective therapy of hypoproteinemia.Methods: SD rats received a single intravenous injection of ADR via tail vein(5.0 mg/kg,7.5 mg/kg,and 10 mg/kg,respectively).After the drug administration,the general condition,urinary production and the onset time of proteinuria were observed.The urinary proteins,serum total protein,serum albumin,serum cholesterol and triglyceride were detected,and the renal pathological examination was performed.The rat model of hypoproteinemia induced by puromycin was served as positive control group.Results: The proteinuria developed on day 4 in 7.5 mg/kg and 10 mg/kg groups.Hypoproteinemia was successfully induced on day 15,with a decrease of serum albumin and total protein and an increase of serum cholesterol and triglyceride.Renal pathological results were consistent with the changes of hypoproteinemia,but the death rate in 10 mg/kg group was high.The similar result was also found in positive control.Conclusion: The rat model of hypoproteinemia can be successfully induced on day 15 after single intravenous injection of ADR at the dose of 7.5 mg/kg.