莲子心生物碱树脂法分离纯化工艺研究

目的 筛选适用于分离纯化莲子心生物碱的大孔吸附树脂。方法 考察吸附性能较好的D101、AB-8、DA201 3种大孔吸附树脂对莲心碱的吸附能力及洗脱参数，并用HPLC定量分析了莲心碱的量。结果 D101树脂对莲子心中生物碱有较好的吸附分离效果。先用水洗除杂，再用6倍树脂体积、乙醇体积分数为80%、pH 3的洗脱剂洗脱，浓缩干燥，产品中莲心碱的质量分数为10.2%、产品收率为24.6%。结论 该工艺简单可行，分离效果好，适合莲子心生物碱的分离纯化。

Separation and purification of alkaloids in Nelumbinis Plumula by resins

Objective To screen the macroporous resin for the separation and purification of alkaloids from Nelumbinis Plumula. Methods The resins such as D101, AB-8, and DA201 were studied for their adsorption capability and elution parameters. The contents of liensinine were determined by HPLC. Results D101 resin had a preferable adsorptive and separative effect for the alkaloids in Nelumbinis Plumula. The preferable desorption ratio could be obtained by using 80% ethanol (pH 3) as eluting solvent and 6 BV. Under these conditions, the content of alkaloids in Nelumbinis Plumula was 10.8% and the yield was 2.6%. Conclusion The method is easy and possesses the popularization potential and good efficacy. It could be used for the separation and purification of alkaloids in Nelumbinis Plumula.