CGRP和ET-1在肝纤维化大鼠心肌组织中的表达及意义

目的　探讨降钙素基因相关肽 (CGRP)和内皮素 - 1(ET- 1)在肝硬化性心肌病 (CCM)发生中的作用。方法　用放免法分别检测肝纤维化大鼠心肌组织中的 CGRP及 ET- 1分布情况。结果　肝纤维化大鼠组 (n=15 )心脏组织中 CGRP浓度 (74.2 130± 10 .3776 pg/ mg蛋白 )和 ET- 1浓度 (1.4780± 0 .92 35 pg/ m g蛋白 )均显著高于对照组 (n=15 ,P<0 .0 5 ) ;且肝纤维化程度越重 ,心肌组织中 ET- 1浓度增加越明显 (P=0 .0 0 4) ;而心脏组织中CGRP浓度并不随肝纤维化程度的加重而增高 (P=0 .30 7)。结论　心肌 CGRP浓度升高可能系机体对 ET- 1及其他致病因子致心脏功能异常的一种保护 /拮抗反应。在 CCM的病理生理过程中 ,CGRP与 ET- 1这两种体液因素间的平衡紊乱可能具有重要的发病学意义。

Alterations in the Level of Calcitonin Gene Related Peptide and Endothelin-1 in the Cirrhotic Rat Heart

Objective　 To investigate the role of calcitonin gene related peptide(CGRP) and endothelin-1(ET-1) in cirrhotic cardiomyopathy (CCM). Methods　We measured the level of CGRP and ET-1 in the samples of rat heart collected from 15 liver cirrhosis rats and 15 controls by using radio immunoassay. Results　 The data showed that the levels of CGRP (74.2130±10.3776pg/mg protein)and ET-1 level(1.4780±0.9235pg/mg protein) were significantly higher in the cirrhotic rat hearts than those in controls (P<0.05). The increase of ET-1 in the cirrhotic rat hearts was closely associated with the severity of liver cirrhosis (P=0.004); whereas no significant association was seen between the CGRP concentration and the severity of liver cirrhosis (P=0.307). Conclusion　We infer that the increasing of CGRP level in the cirrhotic rat heart may be a protective or antagonistic reaction to ET-1 or other pathogenic factors for cardiac dysfunction. The disturbance of the balance between CGRP and ET-1 in the liver cirrhosis rat hearts may contribute to the pathologic process of CCM.