Livin和CyclinD1在慢性萎缩性胃炎伴肠化黏膜中的表达及意义

目的:探讨凋亡抑制蛋白Livin和细胞周期蛋白D1(CyclinD1)在慢性萎缩性胃炎(chronicatrophicgastritis,CAG)伴肠化癌变过程中的表达及其相关性.方法:应用免疫组织化学染色S-P法检测Livin和CyclinD1在30例慢性浅表性胃炎(chronicsuperficialgastritis,CSG)、35例CAG非肠化、35例CAG伴肠化、30例胃癌组织中的表达,并同时研究二者在CAG伴肠化癌变中表达的相关性.结果:在CSG、CAG非肠化、CAG伴肠化、胃癌中Livin、CyclinD1的阳性表达率分别为:0%、10%;28.57%、14.29%;45.71%、37.14%;66.67%、53.33%,二者在CAG(非肠化)和CSG对比均有统计学意义(P<0.05);CAG(伴肠化)与CAG(非肠化)相比,CyclinD1的阳性表达率有统计学意义(P<0.05);Livin和CyclinD1在CAG伴肠化、胃癌组织中表达呈正相关.结论:Livin、CyclinD1蛋白在CAG(伴肠化)和胃癌组织中表达呈上调状态,且CAG(伴肠化)和胃癌组织间无显著差异,二者表达一致,提示CAG(伴肠化)已具有癌变的分子生物学特征.二者在胃癌的发生、发展中起协同作用,对其进行联合检测将有助于胃癌的早期诊断.

Clinical signif cance of expression of livin and cyclin D1 in chronic atrophic gastritis with intestinal metaplasia

AIM:To investigate the expression of livin and cyclinD1 in chronic atrophic gastritis with intestinal metaplasia.METHODS:Immunohistochemistry was used to examine the expression of livin and cyclin D1 in 30 cases of chronic superficial gastritis,35 cases of atrophic gastritis without intestinal metaplasia,35 cases of atrophic gastritis with intestinal metaplasia,and 30 cases of gastric carcinoma.We also explored the correlation between livin and cyclin D1 expression in chronic atrophic gastritis and gastric carcinoma.RESULTS:The positive rates of livin expression in the specimens of chronic superficial gastritis,atrophic gastritis without intestinal metaplasia,atrophic gastritis with intestinal metaplasia,and gastric carcinoma were 0%,28.57%,45.71% and 66.67%,respectively,and the corresponding rates for cyclin D1 expression were 10%,14.29%,37.14% and 53.33%.The positive rates of livin and cyclinD1 expression were significantly higher in atrophic gastritis with intestinal metaplasia than in chronic superficial gastritis(both P < 0.05).The expression of cyclin D1 differed significantly between atrophic gastritis with and without intestinal metaplasia(P < 0.05).The expression of livin has a positive correlation with that of cyclin D1 in atrophic gastritis with intestinal metaplasia and gastric carcinoma.CONCLUSION:The positive rates of livin and cyclin D1 expression increase in atrophic gastritis with intestinal metaplasia and gastric carcinoma.No significant difference was observed for the expression of livin and cyclin D1 between atrophic gastritis with intestinal metaplasia and gastric carcinoma.Livin and cyclin D1 may play a very important role in the development of gastric carcinoma and be used as markers for early diagnosis of gastric carcinoma.