Streptomyces griseus ATCC 13273对去氢骆驼蓬碱的转化研究

利用微生物转化的方法,选取灰色链霉菌(Streptomyces griseus ATCC 13273)为转化菌株,对去氢骆驼蓬碱的转化进行研究。该菌株可以将去氢骆驼蓬碱转化为两种转化产物(记为H1和H2),通过MS、1H-NMR和13C-NMR分析,转化产物分别鉴定为哈尔酚和N-羟基去氢骆驼蓬碱。并对转化条件进行初步优化,优化后的转化条件为:初始pH 8.0,两步活化法以4%的接种量转接,48 h后加入底物(以N,N-二甲基甲酰胺溶解),发酵液中去氢骆驼蓬碱终浓度为0.25 mmoL/L,发酵时间为5 d。转化条件优化后H1和H2的产率分别达到8.70%和12.76%;该研究丰富了S.griseus ATCC 13273的作用底物范围,为哈尔酚和N-羟基去氢骆驼蓬碱的合成提供了一条新途径。

Study on Biotransformation of Harmine by Streptomyces griseus ATCC 13273

Biotransformation studies of harmine by S.griseus ATCC 13273 were conducted.S.griseus ATCC 13273 catalyzed the microbial transformation process of harmine into two metabolites(H1 and H2).The two metabolites were isolated and identified as harmol and N-hydroxyl-harmine by MS,1H-NMR and 13C-NMR analysis.The optimization of harmine biotransformation conditions was carried out and the optimal conditions were determined as follows:pH value 8.0,substrate concentration 0.25mmoL/L,fermentation time 5 d,addition time 48 h,inoculums concentration 4%,and addition mode dissolved in DMF.Under this optimized condition,the yield of harmol and N-hydroxyl-harmine was 8.70% and 12.76%,respectively.This study enriches the range of substrate S.griseus ATCC 13273 catalysis providing a new approach to synthesize harmol and N-hydroxyl-harmine.