| 大鼠海马CA1区神经元P2X受体对ATP、suramin的反应 |
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| 引用本文： | 赵延东,阮怀珍.大鼠海马CA1区神经元P2X受体对ATP、suramin的反应[J].第三军医大学学报,2005,27(16):1674-1677. |
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| 作者姓名： | 赵延东 阮怀珍 |
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| 作者单位： | 第三军医大学基础医学部神经生物学教研室,重庆市神经科学研究所,重庆,400038;第三军医大学基础医学部神经生物学教研室,重庆市神经科学研究所,重庆,400038 |
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| 摘 要： | 目的研究大鼠海马CA1区神经元P2X受体对ATP的反应和suramin、ivermectin(IVM)及低pH值对诱发电流的影响.方法采用酶加机械分离法分离后7 d的Wistar大鼠海马CA1区锥体细胞,用膜片钳全细胞记录技术测定P2X受体激动剂ATP,阻断剂suramin,调节剂ivermectin(IVM)及低pH值(pH=6.5)对跨膜电流的作用.结果分离神经元对P2X受体激动剂和阻断剂反应明显,P2X受体调节剂对不同细胞有不同的作用.结论大鼠海马CA1区锥体细胞有丰富的P2X受体表达.细胞间P2X受体的表达类型有一定差别.
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| 关 键 词： | P2X受体 海马 膜片钳 ATP suramin |
| 文章编号： | 1000-5404(2005)16-1674-04 |
| 收稿时间： | 2004-10-21 |
| 修稿时间： | 2005-01-05 |
Response of P2X receptors in CA1 region of rat hippocampus to ATP and suramin |
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| ZHAO Yan-dong,RUAN Huai-zhen.Response of P2X receptors in CA1 region of rat hippocampus to ATP and suramin[J].Acta Academiae Medicinae Militaris Tertiae,2005,27(16):1674-1677. |
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| Authors: | ZHAO Yan-dong RUAN Huai-zhen |
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| Abstract: | Objective To investigate the effects of ATP, suramin, ivermectin (IVM) and low pH value on pyramidal cells of rat hippocampus. Methods Pyramidal cells were rapidly dissociated from the hippocampus of 7-day postnatal rats by mechanical and enzymatic methods. Effects of ATP, suramin, IVM and low pH value on P2X receptor of neurons were studied by the technique of whole cell patch clamp. Results The transmembrane current was affected by ATP and suramin. There were different effects of IVM and low pH value on ATP induced current on different neurons. Conclusion There is extensive expression of P2X receptor on hippocampus pyramidal cells of rats. The expression of P2X receptors subunits are different among neurons. |
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| Keywords: | ATP suramin |
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