肾母细胞瘤血小板衍化内皮细胞生长因子mRNA的表达及其临床意义

目的探讨小儿肾母细胞瘤中血小板衍化内皮细胞生长因子（PD-ECGF）mRNA的表达及其临床意义。方法用逆转录多聚酶链反应（RTPCR）方法检测30例肾母细胞瘤组织、8例瘤旁、4例无瘤肾组织的PD-ECGF mRNA的表达情况，并分析PD-ECGF mRNA的表达与肾母细胞瘤的病理分型、临床分期、转移及预后之间的关系。结果所有标本均有不同程度的PD-ECGF mRNA的表达，表达程度呈肿瘤组织、瘤旁组织、无瘤组织依次降低。肿瘤组织是瘤旁组织的1．22倍（t=0．855，P〉0．05）；瘤旁组织是无瘤对照组的2．07倍（t=2．65，P〈0．05）；肿瘤组织是无瘤对照组的2．53倍（t=2．05，P〈0．05）。在肾母细胞瘤组织中PD-ECGF mRNA的表达与病理分型无关（F=0．691，P〉0．05），与临床分期也无关（F=0．05，P〉0．05），但有转移者的PD-ECGFmRNA的表达高于无转移者。全部病例获得随访，平均随访50,2个月（17～75个月），术中已发现转移和术后转移复发共18例，死亡7例。所有转移、复发患儿的PD-ECGF mRNA水平是无转移、复发患儿的1．72倍（t=2．2766，P〈0．05）；是无瘤对照组的2．9倍（t=2．608，P〈0．05）。用PD-ECGF mRNA的表达预测肾母细胞瘤转移、复发及死亡的敏感性为83．3％，特异性80．0％。结论PD-ECGF mRNA在肾母细胞瘤组织中呈高表达，与其预后密切相关。提示PD-ECGF mRNA的表达对判断肾母细胞瘤预后有一定的意义。

Expression of platelet-derived endothelial cell growth factor mRNA in Nephroblastoma and its clinical significance

Objective To investigate the mRNA expression of platelet-derived endothelial cell growth factor (PD-ECGF) in Nephroblastoma and its' clinical significance.Methods PD-ECGF mRNA expression were determined by RT-PCR in 30 cases of nephroblastoma tissues and 8 cases of paracancer renal tissues and 4 cases of renal tissue without tumour. The relations about PD-ECGF mRNA expression were also analyzed in nephroblastoma's different clinical stage and different pathology type and metastasis and prognosis. Results Some degree of PD-ECGF mRNA expression was present in all samples. The PD-ECGF mRNA level was 1. 22-fold higher in tumour tissues than in paracancer renal tissues(t = 0. 855,P>0.05) and 2.53-fold higher than in renal tissue without tumour(t=2.05, P< 0. 05) . Paracancer renal tissues expressed 2. 07-fold higher PD-ECGF mRNA level than renal tissue without tumour(t = 2. 65,P<0. 05). We also educe that PD-ECGF mRNA level do not correlate with pathology type(F = 0. 691 ,P>0. 05),clinical stage(F = 0. 05,P>0.05)in nephroblastoma. The most important result we get is that the PD-ECGF mRNA level in nephroblastoma with metastasis is 1. 72-fold higher than the tumour without metastasis(t = 2. 2766,P<0. 05)and is 2. 9-fold higher than the comparision without tumour(t= 2. 608, P<0. 05 ). The sensitivity and specificity for predicting tumour recurrence were 83. 3% and 80. 0% respectively if use 0. 99 as a cutoff value of PD-ECGF mRNA level in this group of nephroblastoma. Conclusions There were over expression of PD-ECGF mRNA in nephroblastoma and the PD-ECGF mRNA level correlates with the tumour's metastasis and recurrence. To analyze the PD-ECGF mRNA level might contribute to the evaluation of nephroblastoma's prognosis.