Immunological detection and definition of minimal residual neuroblastoma disease in bone marrow samples obtained during or after therapy |
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| Affiliation: | 1. Division of Hematology and Oncology, Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA;2. Division of Oncology, Department of Pediatrics, Children''s Hospital of Philadelphia, Pennsylvania, USA;3. Department of Cellular and Molecular Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA;4. Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA;5. Department of Pathology, Children''s Hospital of Philadelphia and University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA;1. Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou & Chang Gung University, Taoyuan, Taiwan;2. Department of Pediatrics, University of California in San Diego, San Diego, CA, United States;1. Livzon Mabpharm Inc, Zhuhai, China;2. State Key Laboratory of Natural Medicines, Department of Chinese Medicines Analysis, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, China;3. AbCyte Therapeutics, Inc, San Jose, California, USA |
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| Abstract: | Immunological staining by the alkaline phosphatase/anti-alkaline phosphatase (APAAP) technique has been used to recognize low levels of neuroblastoma cells in bone marrow mononuclear cells. Immunological phenotyping with 11 well characterized monoclonal antibodies was performed on 16 children with neuroblastoma and BM involvement during or after therapy. Neuroblasts were detected in 11 of 16 patients (0.1–5%), whereas BM biopsies on six of these patients were classified as normal. Aspirates, stained conventionally, were positive for pathological cells in three patients only.The comparison of the phenotype of the neuroblastoma cells at the time of diagnosis to the phenotype of the residual cells within one patient revealed differences. The phenotype of residual disease in different patients on the other hand showed a unique pattern. The above mentioned results lead to the conclusion that the immunological procedure is particularly suitable for the analysis of minimal residual neuroblastoma since the technique allows very minor cell populations to be identified in BM samples. |
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