Developmental changes of glutamate receptors in the rat cerebral cortex and hippocampus |
| |
Authors: | Y Arai Masashi Mizuguchi and Sachio Takashima |
| |
Institution: | (1) Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, National Center for Neurology and Psychiatry, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187, Japan Tel.: +81-423-46-1713; Fax: +81-423-46-1743, JP |
| |
Abstract: | We studied the immunohistochemial localization of the glutamate receptors (GluR-1, -2, and -3,) in the developing rat cerebral
cortex and hippocampus using antibodies to GluR1 and to an epitope common to GluR2 and GluR3 (GluR2/3) subunits. In the cerebral
cortex, GluR1 immunoreactivity appeared in the neurons from postnatal day (PND) 0, increased with maturation, was highest
at PND 10, decreased until PND 30, and thereafter remained at the same level as on PND 0. GluR2/3 immunoreactivity appeared
earlier in scattered neurons on embryonal day (ED) 18, increased with maturation and reached a peak between PND 10 and PND 15,
after which the immunoreactivity gradually decreased and reached a plateau at PND 30. For both GluR1 and GluR2/3, some of
the pyramidal neurons showed intense staining. In the pyramidal layers of the hippocampus, GluR1 and GluR2/3 immunoreactivity
was found in all the pyramidal neurons of the CA1–4 area from ED 20. In the dentate gyrus of the hippocampus, GluR1 and GluR2/3
immunoreactivity was found in the neurons of the granule cells after PND 0. Immunoreactivity in the neurons of the subiculum
was found after PND 5 and that of the polymorphic cell layers was found after PND 15–20. Our results indicate that the development
of glutamate receptor subunits in the rat cerebral cortex and hippocampus is expressed in different spatial patterns and distinct
temporal patterns throughout development and is scheduled during the early postnatal period, when synaptic plasticity or synaptic
connection occurs in these regions.
Accepted: 13 June 1996 |
| |
Keywords: | α -Amino-3-hydroxy-5-methyl-4-isoxazole proprionic acid receptor Glutamate receptor development Immunohistochemistry Synaptogenesis Rat |
本文献已被 SpringerLink 等数据库收录! |
|