PD-1/B7-H1 Interaction Contribute to the Spontaneous Acceptance of Mouse Liver Allograft |
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| Authors: | M Morita M Fujino G Jiang Y Kitazawa L Xie M Azuma H Yagita S Nagao A Sugioka Y Kurosawa S Takahara J Fung S Qian L Lu X-K Li |
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| Institution: | Education and Research Center of Animal Models for Human Diseases, Fujita Health University School of Medicine, Aichi, Japan;Department of Surgery, Fujita Health University School of Medicine, Aichi, Japan;Division of Immunology, Institute for Comprehensive Medical Science, Fujita Health University School of Medicine, Aichi, Japan;Laboratory of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan;AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan;Department of Immunology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH;Department of General Surgery, Lerner Research Institute, Cleveland Clinic, Cleveland, OH;Department of Advanced Technology for Transplantation, Osaka University Graduate School of Medicine, Osaka, Japan;Department of Molecular Immunology, Graduate School, Tokyo Medical and Dental University, Japan;Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan |
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| Abstract: | The programmed death-1 (PD-1)/B7-H1 pathway acts as an important negative regulator of immune responses. We herein investigated the role of the PD-1/B7-H1 pathway in establishing an immunological spontaneous tolerance status in mouse liver allografting. B7-H1 is highly expressed on the donor-derived tissue cells and it is also associated with the apoptosis of infiltrating T cells in the allografts. Strikingly, a blockade of the PD-1/B7-H1 pathway via anti-B7-H1mAb or using B7-H1 knockout mice as a donor led to severe cell infiltration as well as hemorrhaging and necrosis, thus resulting in mortality within 12 days. Furthermore, the expression of the FasL, perforin, granzyme B, iNOS and OPN mRNA in the liver allografts increased in the antibody-treated group in comparison to the controls. Taken together, these data revealed that the B7-H1 upregulation on the tissue cells of liver allografts thus plays an important role in the apoptosis of infiltrating cells, which might play a critical role of the induction of the spontaneous tolerance after hepatic transplantation in mice. |
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| Keywords: | Apoptosis B7-H1 orthotopic liver transplantation regulatory cell spontaneous tolerance |
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