地锦草总黄酮预防四氯化碳所致急性肝损伤的实验研究

目的研究地锦草总黄酮(total flavonoids ofEuphorbiae hum ifusae,TFEH)对四氯化碳造成的小鼠急性肝损伤的保护作用。方法四氯化碳造成小鼠急性肝损伤模型,测定血清谷丙转氨酶(serum glutam ic pyruvic transam inase,SGPT)、肝组织谷丙转氨酶(liver glutam ic pyruvic transam inase,GPT)、超氧化物歧化酶(superoxidase d ismutase,SOD)活性及丙二醛(m alonaldehyde,MDA)、还原性谷胱甘肽(GSH)含量,采用光镜观察肝脏组织学变化。结果与四氯化碳模型组比较,经TFEH预防性治疗后,GPT、LGPT活性和MDA含量均明显降低(P<0.05或P<0.01),而GSH含量和SOD活力明显增高(P<0.05)。通过病理学切片观察,TFEH明显改善肝组织的病理变化。结论TFEH对四氯化碳造成的小鼠急性肝损伤具有保护作用。

Protective Effect of Total Flavonoids of Herba Euphorbiae humifusae against Hepatic Injury Induced by CCl4 in Mice

Objective The protective effect of total flavonoids Euphorbiae humifusae against CCl_4-induced hepatic injury in mice was observed.Methods Acute liver injury model in mice was made by using CCl_4.The levels of SGPT,LGPT in serum and MDA,SOD,GSH in liver and hepatic pathological tissue were observed.Results Compared with model control group,the levels of serum SGPT,LGPT,liver MDA of the mice treated with TFEH significantly decreased(P<0.05 or P<0.01),while the activity of SOD and the level of GSH increased greatly(P<0.05).By observation of pathological slices,TFEH could improve hepatic pathological changes distinctly.Conclusion TFEH can prevent CCl_4-induced hepatic injury.