丹参注射液及血栓通注射液对缺血再灌注大鼠肾脏的影响

本文研究了丹参注射腋（ＳＭ）及以三七皂甙为主要成分的血栓通注射液（ＡＲ）对缺血再灌注大鼠肾脏的影响。将Ｗｉｓｔａｒ大鼠肾蒂夹闭造成缺血１ｈ再灌注前１０ｍｉｎ分别给大鼠尾静脉注射ＳＭ及ＡＲ，于再灌注１５ｍｉｎ测定肾皮质ＳＯＤ，ＧＳＨｐｘ活力及ＧＳＨ，ＧＳＳＧ含量。于再灌注２４ｈ后检查大鼠血肌酐含量，并进行病理学检查。结果发现，ＳＭ和ＡＲ起到了相当于活性氧清除剂的作用，显著地改善了大鼠肾脏的再灌注性损伤，无论是肾脏的功能或形态均较未用药组有显著的改善；肾皮质ＳＯＤ及ＧＳＨｐｘ的活力亦受到有效的保护；肾皮质ＧＳＨ含量显著低于未用药组。

The effect of Salviae Miltiorrhizae andArasaponin on ischemia-reperfusion rat kid-ney

any Chinese herbs and their extractshave been proved to be effective reactiveoxygen metabolites (ROM) scavengers. Inthis study, we investigated the effect ofSalviae Miltiorrhizae (SM) and Arasaponin(AR) , as ROM scavengers , on ischemic-reperfusion rat kidney. Wistar rats wereused. The right kidney was removed ,the leftrenal pedical was clarnped for I hour, thenthe clamp was released for reperfusion(IR). SM and AR were respectively injectediv. 10 min before reperfusion in differentgroups. Renal cortex (RC) SOD , GSHpx ,GSH and GSSG were determined after 15min reperfusion- Serum creatinine (Scr) wasdetermined after 24 h reperfusion. Kidneyspecimens were examined under light micro-scope (LM) and electron microscope (EM) .LM and EM showed that SM and AR couldalleviate greatly the pathological injury in-duced by ischemia-reperfusion. Elevation ofScr was significantly reduced than that ofLR rats. The decrease of RC SOD and GSH-px activity induced by 15 rnin reperfusionwas ameliorated greatly too. However, intreated rats ,RC GSH was lower than that inIR rats ,redox index was higher than that inLR rats. Our data suggested that SM andAR had ROM scavenger-like effect. Thusthey could ameliorate ischernic-reperfusionrenal injury.