SOCS-1和SOCS-3在慢性根尖周炎病损组织中的表达及临床意义

目的:检测细胞因子信号转导抑制因子1 (suppressor of cytokine signaling-1,SOCS-1)和细胞因子信号转导抑制因子3 (suppressor of cytokine signaling-3,SOCS-3)在慢性根尖周炎病损组织中的表达水平,探讨SOCS-1、3在慢性根尖周炎发病机制中的作用.方法:收集25例慢性根尖周炎病损组织作为病例组,16例健康牙的牙周膜组织作为对照组.对样本中的蛋白表达量采用免疫组织化学法检测,mRNA表达量采用实时定量PCR法检测.采用SPSS13.0软件包对数据进行统计学分析.结果:免疫组织化学结果显示,SOCS-1、3蛋白在慢性根尖周炎病损组织中的表达水平均显著高于对照组(P＜0.01);重度炎症组中,SOCS-1、3的蛋白表达显著高于轻中度炎症组(P＜0.01).SOCS-1mRNA在轻中度炎症组、重度炎症组和对照组中的表达量分别为2.620±1.552、2.373±1.083和1.277±1.040,SOCS-3mRNA的表达量分别为9.308±5.901,7.565±3.233和1.232±1.099.SOCS-1、3 mRNA在轻中度炎症组、重度炎症组均显著高于对照组(P＜0.01),但不同炎症组之间并无显著差异.结论:SOCS-1、3可能与慢性根尖周炎密切相关.

Expression of SOCS-1 and SOCS-3 in chronic periapical lesions and its clinical significance

PURPOSE:To detect the expression of suppressor of cytokine signaling-1 (SOCS-1) and suppressor of cytokine signaling-3 (SOCS-3) in chronic periapical lesions and to clarify their roles in pathogenesis of apical periodontitis.METHODS:A total of 25 chronic periapical lesion tissues and 16 normal periodontal ligament tissues were collected respectively.The expression of mRNA was measured by real-time PCR,the protein expression was measured by immunohistochemical analysis.Statistical analysis was performed using SPSS 13.0 software package.RESULTS:Immunohistochemical results indicated that expression levels of SOCS-1 and SOCS-3 protein in chronic periapical lesions were significantly higher than that in normal tissues (P＜0.01);Moreover,SOCS-1 and SOCS-3 protein expression levels in severe inflammation group were significantly higher than that in mild inflammation group (P＜0.01).In mild inflammation group,severe inflammation group and control group,the expression levels of SOCS-1 mRNA were 2.620±1.552,2.373± 1.083 and 1.277±1.040,whereas those of SOCS-3 were 9.308 ±5.901,7.565 ±3.233 and 1.232 ±1.099,respectively.CONCLUSIONS:The expression levels of SOCS-1 mRNA and SOCS-3 mRNA are significantly higher both in mild inflammation group and severe inflammation group than that in control group (P＜0.01),while no significant difference is found in mild and severe inflammation group.