低频超声联合曲安奈德对金黄地鼠颊黏膜溃疡愈合的影响

目的:研究低频超声联合曲安奈德对金黄地鼠实验性口腔溃疡愈合时间,以及溃疡组织中超氧化物歧化酶(superoxide dismutase,SOD)和微量丙二醛(malondialdehyde,MDA)含量的影响.方法:将60只金黄地鼠随机分为5组,1组为基线组(包括正常基线组和溃疡基线组,各6只),另外4组为实验组(低频超声+药物组、单独超声组、单独药物组和空白对照组),每组12只,各组通过“氧自由基损伤法”建立口腔溃疡模型.末次处理24 h后观察溃疡愈合情况,并检测溃疡组织中的SOD活性及MDA含量.采用SPSS 20.0软件包对数据进行统计学分析.结果:低频超声+药物组较单独超声组、单独药物组及空白对照组溃疡愈合时间显著缩短.末次处理24 h后SOD检测结果显示,低频超声+药物组(2.32±0.30) U/mgprot]较溃疡基线组(1.48±0.29) U/mgprot]、单独超声组(1.83±0.15)U/mgprot]、单独药物组(1.76±0.25) U/mgprot]及空白对照组(1.71±0.18) U/mgprot]显著增高(P＜0.05);低频超声+药物组MDA含量(8.17±0.21) nmol/mgprot]较溃疡基线组(9.41±0.22)nmol/mgprot]、单独超声组(9.00±0.44) nmol/mgprot]、单独药物组(9.04±0.43) nmol/mgprot]及空白对照组(9.03 ±0.46) nmol/mgprot]显著降低(P＜0.05).溃疡愈合后SOD活性及MDA含量检测显示,低频超声+药物组、单独超声组、单独药物组及空白对照组与正常基线组相比无显著差异(P＞0.05).结论:低频超声协同曲安奈德能显著缩短溃疡愈合时问,增加溃疡组织中SOD活性,降低MDA含量,对溃疡的愈合有一定的促进作用.

Effect of low-intensity pulsed ultrasound combined with triamcinolone acetonide on oral mucosal ulcer healing in syrian hamster

PURPOSE:To evaluate the effect of low-intensity pulsed ultrasound (LIPUS) combined with triamcinolone acetonide on oral mucosal ulcer in syrian hamster in several ways,including healing time,contents of superoxide dismutase (SOD)and malondialdehyde (MDA).METHODS:Sixty syrian hamsters were randomly divided into 5 groups,including a baseline group (containing a normal baseline group and a model baseline group,n=6) and 4 experimental groups (LIPUS processing and drug use group,LIPUS group,drug group and a normal control group without any processing,n=12).Four experimental groups and model baseline group were given oxygen free radicals to model the oral mucosal ulcer.At 24 h after the last treatment,the healing time of ulcer,content of SOD and MDA were compared between each group.SPSS 20.0 software package was used for statistical analysis.RESULTS:Compared with LIPUS group,drug group and control group,the healing time of oral mucosal ulcer in LIPUS and drug combined group was shortened.At 24 h after the last treatment,the activity of SOD showed that the LIPUS and drug combined group (2.32± 0.30) U/mgprot] were significantly higher than the model baseline group (1.48±0.29) U/mgprot],the LIPUS group (1.83± 0.15) U/mgprot],the drug group(1.76±0.25) U/mgprot] and control group(1.71±0.18) U/mgprot] (P＜0.05).The results of MDA content showed that the LIPUS and drug combined group (8.17±0.21) nmol/mgprot] were significantly lower than the model baseline group(9.41 ±0.22) umol/mgprot],the LIPUS group(9.00±0.44) nmol/mgprot],the drug group (9.04±0.43) nmol/mgprot] and control group (9.03±0.46) nmol/mgprot] (P＜0.05).After oral mucosal ulcer healing,the activity of SOD and MDA showed that the LIPUS and drug combined group,the LIPUS group,the drug group and control group were not significantly different from the normal baseline group (P＞0.05).CONCLUSIONS:Low-intensity pulsed ultrasound combined with triamcinolone acetonide can effectively improve the activity of SOD and reduce the contents of MDA in ulcerated tissues,and therefore accelerate the process of ulcer healing.