Smad4、Smad7和Caveolin-1在Wistar大鼠口腔黏膜癌变中的表达和意义

目的:研究母亲DPP同源物4 (mothers against decapentaplegic homolog 4,Smad4)、母亲DPP同源物7(mothers against decapentaplegic homolog 7,Smad7)和小窝蛋白1 (Caveolin-1)在Wistar大鼠口腔黏膜癌变过程中的表达,了解TGF-β/Smad信号传导通路和小窝蛋白Caveolin-1在口腔癌前病变中的作用.方法:采用郑州大学口腔医学院存档的Wistar大鼠口腔腭部黏膜癌变标本,包括正常黏膜5例,单纯增生黏膜10例,轻度异常增生黏膜6例,中度异常增生黏膜7例,重度异常增生黏膜13例,口腔癌变组织28例.应用免疫组织化学SP法检测Smad4、Smad7和Caveolin-1蛋白的表达,采用SPSS 15.0软件包对数据进行统计学分析.结果:Smad4蛋白在正常和单纯增生黏膜、上皮异常增生黏膜和口腔癌黏膜中表达依次降低,3组间差异显著(P＜0.05);Smad7和Caveolin-1蛋白在正常和单纯增生黏膜、上皮异常增生黏膜和口腔癌黏膜中表达均依次升高,3组间差异显著(P＜0.05).经Spearman相关分析,Smad4与Smad7蛋白表达呈负相关,Smad4与Caveolin-1蛋白表达呈负相关,Smad7与Caveolin-1蛋白表达呈正相关(P＜0.05).结论:Smad4、Smad7和Caveolin-1蛋白在口腔黏膜癌变过程中可能存在协同作用.

Significance of Smad4, Smad7 and Caveolin-1 expression in oral mucosa carcinogenesis of Wistar rats

PURPOSE:To investigate the expression of Smad4,Smad7 and Caveolin-1 in the process of carcinogenesis of oral mucosa in Wistar rats,and to understand the changes of TGF-β/Smad signaling pathway and Caveolin-1 in oral cancer.METHODS:Palatal mucosal carcinogenesis specimens of Wistar rats were obtained from School of Stomatology,Zhengzhou University,which included 5 samples of normal mucosa,10 samples of simple hyperplasia mucosa,6 samples of mild mucosal dysplasia,7 samples of moderate mucosal dysplasia,13 samples of mucosa severe mucosal dysplasia,and 28 samples of oral cancer tissue.The expression of Smad4,Smad7 and Caveolin-1 was detected by immunohistochemistry.SPSS15.0 software package was used for statistical analysis.RESULTS:The expression of Smad4 decreased in normal and hyperplastic epithelia,dysplasticepithelia and oral cancer gradually,the difference of the expression among the three groups was significant (P＜0.05).The expression of Smad7 and Caveolin-1 increased in normal and hyperplastic epithelia,dysplasticepithelia and oral cancer gradually,respectively;the difference of the expression among the 3 groups was significant (P＜0.05).Spearman correlation analysis showed that Smad4 was negatively correlated with Smad7,Smad4 was negatively correlated with caveolin-1,Smad7 was positively correlated with Caveolin-1 (P＜0.05).CONCLUSIONS:Synergistic effects may exist among Smad4,Smad7 and caveolin-1 in carcinogenesis of oral cancer.