| 乙型肝炎病毒多表位治疗性抗原基因的合成、表达及抗原性研究 |
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| 引用本文： | 骆利敏,李明,夏虎,黄建生,陈百虹,王萍. 乙型肝炎病毒多表位治疗性抗原基因的合成、表达及抗原性研究[J]. 南方医科大学学报, 2003, 23(8): 802-805 |
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| 作者姓名： | 骆利敏 李明 夏虎 黄建生 陈百虹 王萍 |
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| 作者单位： | 1. 第一军医大学热带病研究所,广东,广州,510515
2. 第一军医大学珠江医院呼吸科,广东,广州,510282 |
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| 基金项目： | 国家自然科学基金(30170532)，广东省自然科学基金(990375)，广州市重点攻关课题(2001-2-035-01)~~ |
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| 摘 要： | 目的研究乙型肝炎病毒多表位治疗性疫苗基因的合成、表达及其产物的抗原性。方法设计、并合成乙型肝炎病毒多表位抗原基因BPT,克隆入融合表达载体pWR450-1,构建重组质粒PWR/BPT。在大肠杆菌中表达乙型肝炎多表位抗原蛋白并纯化该蛋白,并用Western-blotting方法初步检测该抗原蛋白的抗原性。结果成功构建了融合表达载体PWR/BPT,在大肠杆菌中表达出乙型肝炎多表位抗原蛋白,Western-blotting 检测显示该蛋白具有良好抗原性。结论HBV多表位治疗性抗原基因的设计是成功的,其在大肠杆菌中表达的重组融合蛋白具有良好的抗原性,可能是较理想的HBV治疗性疫苗候选物。
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| 关 键 词： | 乙型肝炎 多表位 治疗性疫苗 |
| 文章编号： | 1000-2588(2003)08-0802-04 |
| 修稿时间： | 2003-04-19 |
Synthesis, cloning, expression and antigenicity of the therapeutic multi-epitope gene of hepatitis B virus and of its products |
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| LUO Li-min ,LI Ming ,XIA Hu ,HUANG Jian-sheng ,CHENG Bai-hong ,WANG Ping Institute of tropic diseases,First Military Medical University,Guangzhou ,China. Synthesis, cloning, expression and antigenicity of the therapeutic multi-epitope gene of hepatitis B virus and of its products[J]. Journal of Southern Medical University, 2003, 23(8): 802-805 |
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| Authors: | LUO Li-min LI Ming XIA Hu HUANG Jian-sheng CHENG Bai-hong WANG Ping Institute of tropic diseases First Military Medical University Guangzhou China |
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| Affiliation: | LUO Li-min 1,LI Ming 1,XIA Hu 2,HUANG Jian-sheng 1,CHENG Bai-hong 1,WANG Ping 11 Institute of tropic diseases,First Military Medical University,Guangzhou 510515,China, 2 Department of Respiratory Diseases,Zhujiang Hospital,First Military Medical University,Guangzhou 510282,China |
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| Abstract: | Objective To study the synthesis, cloning, expression and antigenicity of therapeutic multi-epitope gene of hepatitis B virus. Methods The therapeutic multi-epitope gene of hepatitis B virus was synthesized and cloned into the vector pWR450-1, then was expressed in E.coli and the products were purified. The immunogenicity of the expressed protein was analyzed by Western-blotting. Results Recombinant plasmid PWR/BPT was constructed successfully and the protein of multiepitope gene of hepatitis B virus was expressed in E.coli, which showed ideal antigenicity by Western-blotting. Conclusions The designed multi-epitope therapeutic gene of hepatitis B virus was proved to be correct and the expressed protein may be a good therapeu- tic vaccine. |
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| Keywords: | hepatitis B virus multi-epitope therapeutic vaccine |
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