防己黄芪汤加味治疗大鼠脊髓半横断损伤

目的：研究中药复方对急性脊髓半切损伤的治疗疗效及对脊髓组织病理改变及星型胶质细胞的影响,并探讨其作用机制。方法：65只大鼠随机取5只作为空白组,余60只大鼠脊髓损伤造模后随机分为损伤、中药和甲基强的松龙三组,各20只。损伤组造模后不用药,中药组造模后予中药治疗,甲基强的松龙组造模后予甲基强的松龙治疗。于术后24小时、1、2、3、4周观察三组大鼠脊髓运动功能恢复、光密度测定及脊髓组织学改变。结果：中药组及甲基强的松龙组脊髓运动功能及光密度评分与损伤组相比有统计学意义（P〈0.01）;中药组病变与损伤组相比,病损缩小、空泡面积减少。中药组与甲基强的松龙组后期脊髓运动功能评分无统计学差异（P〉0.05）。结论：中药组在改善脊髓微环境、保护神经组织免受损害、促进间质细胞生长上发挥其有益作用,抑制胶质细胞的过度增生,从功能和形态学上证实了中药治疗的有效性。

Empirical study on Fangjifangqi decoction in treating Hemitransected Spinal Cord Injury in Rats

Objective:To evaluate the therapeutic effect of Fangjifangqi decoction in treating acute experimental hemitransected spinal cord injury and discuss its active mechanism by observing the pathological and astrocyte changes of spinal cord. Methods:Of 65 SD rats,5 SD rats were randomly divided into control group and other 60 SD rats were made into hemitransected spinal cord injury model by hemitransection method, then randomly divided into trauma group, TCM group and MPs group. The function recovery of spine cord, light density grade and pathological changes of spinal cord were observed at 24h,1w,2w,3w,4w respectively after the operation. Results:The function recovery of spine cord and light density grade in TCM group and MPs group were better than trauma group, which had statistical significance (P<0.01).The trauma in nerve tissue was reduced and the vacuole area was diminished in spine cord in the TCM group. The difference of spine cord function recovery between TCM group and MPs group had no statistical significance two weeks later (P>0.05). Conclusion:The TCM treatment can promote the microenvironment of spine cord, protect the nerve tissue and restrain the astrocyte excess hyperplasia, which was proved by the functional evaluation and morphologic observation in the study.