Effect of cold-dryness on pulmonary and immunologic function in chronic obstructive pulmonary disease model rats |
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Authors: | Zhen Gao Fengsen Li Halmurat Upur Jiang Min Wang Jing Jing Jing Dan Xu |
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Affiliation: | 1. Dan Xu, National Clinical Research Base of Traditional Chinese Medicine, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University, Urumqi 830000, China;2. National Clinical Research Base of Traditional Chinese Medicine, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University, Urumqi 830000, China;3. Institute of Traditional Uighur Medicine, Xinjiang Medical University, Urumqi 830011, China |
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Abstract: | ObjectiveTo study the effects of cold-dryness on pulmonary and immunologic function of peripheral T-lymphocytes in chronic obstructive pulmonary disease (COPD) model rats, and to provide references for the prevention and treatment of cold-dryness COPD in the Xinjiang region.MethodsThe COPD model was established with an elastase drip into the trachea combined with smoking. The cold-dryness COPD model was developed by stressing with a cold-dry environment. Success of the model was determined by observation of pathologic lung sections. Rats were sacrificed by exsanguination from the femoral artery and changes of peripheral blood CD4+, CD8+, and CD4+/CD8+ were detected by flow cytometry. Data were analyzed with SAS 11.5 statistical software.ResultsOn the ninetieth day after ending the experiment, Peak expiratory flow in the cold-dryness COPD group was lower than that in the COPD and normal control groups (P<0.01). The time of inspiration in the cold-dryness COPD group was higher than that in the COPD and normal groups (P<0.05). Time of expiration (Te) in the cold-dryness COPD group was higher than that in the COPD and normal groups (P<0.01). 50% tidal volume expiratory flow (EF50) in the cold-dryness COPD group was lower than that in the COPD and normal groups (P<0.01), and EF50 in the COPD group was lower than that in the normal group (P<0.05). CD4+ content of peripheral blood in the cold-dryness COPD group was lower than that in the COPD and the normal groups (P<0.05). CD8+ content in the cold-dryness COPD and COPD groups was higher than that in the normal control group (P<0.01), and CD8+ content in the cold-dryness COPD group was higher than that in the COPD group (P<0.01). CD4+/CD8+ in the cold-dryness COPD group and the COPD group was lower than that in the normal control group (P<0.01), and CD4+/CD8+ in the cold-dryness COPD group was lower than that in the COPD group (P<0.05).ConclusionIn the cold-dryness COPD model, CD8+ increased and CD4+/CD8+ decreased. Moreover, cold-dryness may aggravate this state. The effects of cold-dryness on pulmonary function mainly manifested as prolongation of Te and decrease of EF50, which could be one of causes of cold-dryness environment in the northwest of China leading to COPD with region characteristics. |
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Keywords: | Pulmonary disease, chronic obstructive T-lymphocytes CD4-CD8 ratio Cold-dryness |
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