| 幼年和成年大鼠外周血T细胞亚群变化 |
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| 引用本文： | 葛依工,高瀚,孔宪涛,张鲁榕,张浩. 幼年和成年大鼠外周血T细胞亚群变化[J]. 第二军医大学学报, 1988, 0(4) |
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| 作者姓名： | 葛依工 高瀚 孔宪涛 张鲁榕 张浩 |
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| 作者单位： | 长医征院普外科(葛依工，高瀚)，长征医院临床免疫中心(孔宪涛，张鲁榕)，长征医院临床免疫中心(张浩) |
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| 摘 要： | 本实验应用了MAbs和流式细胞仪做为检测方法,比较观察了不同鼠龄的大鼠外周血T细胞亚群分布变化。结果显示,幼鼠(40d)和成鼠(5～6个月)的外周血T细胞亚群变化有明显差异:成鼠的OX-19~+(全T细胞)和W3/25~+(Th/i)细胞细胞较幼鼠有明显减少(P<0.001),而OX-8~+(Ts/C细胞)亚群却较幼鼠明显升高。但W3/25~+和OX- 8+亚群细胞表面的荧光密度变化却与阳性细胞百分率相反。推测造成这种T细胞亚群变化的原因可能与T细胞的发育成熟及T细胞本身的调节有关。实验资料为研究大鼠T细胞亚群的发育成熟过程提供了重要数据。
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| 关 键 词： | 外用血T细胞亚群 单克隆抗体 流式细胞仪 大鼠 |
PERIPHERAL BLOOD T LYMPHOCYTE SUBSETS IN CHILD AND ADULT RATS: A COMPARATIVE STUDY |
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| Ge Yigong,. PERIPHERAL BLOOD T LYMPHOCYTE SUBSETS IN CHILD AND ADULT RATS: A COMPARATIVE STUDY[J]. Former Academic Journal of Second Military Medical University, 1988, 0(4) |
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| Authors: | Ge Yigong |
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| Abstract: | A comparative study was made for evaluation of peripheral blood T lymphocyte subsets in child (40 days) and adult (5-6 months)rats.There was significant difference between child and adult rats in peripheral blood T cell subsets: in the child, there were more OX-l9+(pan T cell)and W3/25+(Th/i cell) cells, and less OX-8+ (Ts/c cell)cells as compared to the adult. In W3/25+ and OX-8+ subsets, however, the fluorescence intensity of cell surface in FCM histogram presented oppositc trend in comparison with the percentage of these cells. It is assumed that the reason resulting in such change is either T cell maturation of the down regulation of T cell antigen receptors.The data described here are provided with some important information for further study in rat T cell maturation. |
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| Keywords: | T lymphocyte subpopulatjons cytornetry monoclonal antibody rats |
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