载脂蛋白E基因多态性与早发冠心病遗传关系研究

目的探讨载脂蛋白E（apoE）基因多态性与早发冠心病（CHD）的关系。方法应用聚合酶链-限制性片段长度多态性（PCR—RFLP）方法先扩增出apoE基因第4外显子内的227bp片段，继以限制性内切酶CfoI酶解消化，8％聚丙烯酰胺凝胶电泳，最后银染显带判定基因型，并计算等住基因频率。共检测了52例早发冠心病患者和110例正常对照者的apoE基因型及等位基因频率。结果早发冠心病患者apoEε4／3基因型和ε4等位基因频率显著高于正常对照组（P〈0．05）。结论apoE基因多态性与早发冠心病的发生有关。该检测方法快速、准确、分辨率高。

Analysis of relationship between apolipoprotein E gene polymorphism and early onset of coronary heart disease

Objective To investigate the relationship between appolipoprein E （apoE） gene polymorphism and early - onset coronary heart disease. Methods The 277 base DNA fragment in the forth exon of apoE gene was amplified by using polymerase chain reaction- restriction fragmant length polymorphism and then digested with restriction enzyme CfoI, eleetrophoresis on 8% polyacrylamaid gel for the cleavaged products, subsequently genotype was determined by silver staining and allele frequencies was analyzed in 52 eases with early - onset coronary heart disease and 110 healthy controls. Results The hererozygous genotyping apoEε4/3 and the frequencies ofε4 allele in early - onset coronary heart disease group were significantly higher than that in age - matched controls （P 〈 0.05）. Conclusion The occurrence of early- onset CHD is affected by the apo E gene polymorphisms.