RhoE表达上调抑制U87细胞增殖与侵袭

目的探讨RhoE在胶质瘤发生发展过程中的作用。方法将包含RhoE基因的质粒转染U87细胞后，采用四唑盐（MTr）法检测细胞增殖能力的变化；Transwell侵袭试验检测细胞侵袭能力的改变；Westernblot分析细胞基质金属蛋白酶（MMP）-2和-9的表达变化。结果RhoE蛋白在U87和U251细胞系中表达明显低于正常人脑胶质细胞；上调RhoE表达，U87细胞增殖和侵袭能力明显下降（P〈0．05）；Westernbolt检测显示上调RhoE表达能够降低U87细胞MMP-2和MMP-9蛋白表达水平（P〈0．05）。结论上调RhoE的表达能够抑制胶质瘤U87细胞的增殖和侵袭能力。

Up-regulation of RhoE expression inhibits cell proliferation and invasion of glioma U87 cells

Objective To evaluate the roles of RhoE in the tumorgenesis and development of glioma. Methods Cell proliferation and invasion of glioma U87 cells infected by Full-length RhoE vector were detected by methyl thiazolyl tetrazolium （M＇IT） assay and Boyden Chamber assay. Matrix-metalloproteinase （MMP）-2 and MMP-9 were determined by Western blot. Results Expression of RhoE in U87 and U251 cells was lower compared to normal human astrocytes; up-regulated RhoE protein significantly inhibited the invasion of U87 cells （ P 〈 0. 05 ）. Up- regulated RhoE reduced the expression of MMP-2 and MMP-9 （P 〈 0. 05）. Conclusion Up-regulated RhoE can inhibit the proliferation and invasion of glioma U87 cells.