VEGF和TIMP—1表达与乳腺癌浸润、淋巴结转移关系的探讨

目的 研究乳腺癌组织中血管内皮生长因子（VEGF）和金属蛋白酶组织抑制因子（TIMP－1）的表达变化与乳腺癌生物学行为及淋巴结转移的关系。方法 应用S－P免疫组化方法检测85例乳腺癌组织VEGF、TIMP－1及细胞增值核抗原Ki－67的表达情况。结果 VEGF阳性染色率为88％，VEGF阳性表达与肿瘤浸润、淋巴结转移、TNM分期及Ki－67指数呈正相关（x^＝7．31，P＜0．05，x^＝4．59，P＜0．05，x^＝10．71，P＜0．01，x^＝7．04，P＜0．05）。TIMP－1阳性染色率为79％，TIMP－1阳性表达与肿瘤浸润、淋巴结转移及TNM分期呈负相关（x^＝12．81，P＜0．01，x^＝4．94，P＜0．05，x^＝9．9，P＜0．05）。VEGF和TIMP－1联合表达与乳腺癌浸润转移及Ki－67指数显著相关（x^＝13．09，P＜0．01，x^＝16．64，P＜0．001，x^＝8．63，P＜0．05）。结论 VEGF高表达和TIMP－1低表达在乳腺癌浸润、淋巴结转移及细胞增殖中起重要作用。

Breast Cancer with VEGF Overexpressive and TIMP-1 Low-expression has High Potential for Tumor Invasion and Lymph Node Metastasis

Objective To study correlation between the expression of VEGF and TIMP 1 and biology behavior and lymph node metastasis.Methods The expression of VEGF,TIMP 1 and Ki 67 were detected with S P immunohistochemical method in 85 breast cancers.Results The positive rate of VEGF expression is 88%.The expression of VEGF is positively related to the tumor invasion,lymph node metastasis,TNM stage and index of Ki 67.(x 2=7 31 P<0 05,x 2=4 59 P<0 05,x 2=10 71 P<0 01,x 2=7 04 P<0 05 respectively).The positive rate of TIMP 1 expression is 79%.A significant negative correlation was found between the expression TIMP 1 and the tumor invasion,lymph node metastasis and TNM stage(x 2=12 81 P<0 01,x 2=4 94 P<0 05,x 2=9 9 P<0 05).Overexpression of VEGF and low expression of TIMP 1 are remarkably related to tumor invasion,metastasis and index of Ki 67(x 2=13 09 P<0 01,x 2=14 64 P<0 001,x 2=8 63 P<0 05 respectively)Conclusion It is suggested that VEGF overexpressive and TIMP 1 low expression might play a significant role in lymph node metastasis,tumor invasive and proliferation in breast cancer.