羊栖菜多糖体外诱导人大肠癌细胞凋亡

目的研究羊栖菜多糖(SFPS)对人大肠癌lovo细胞和RKO细胞增殖的作用及其意义。方法MTT法检测SFPS在体外抑制大肠癌细胞增殖的抑制率;扫描电镜、透射电镜和流式细胞术鉴定细胞凋亡。结果SFPS对lovo细胞和RKO细胞具有显著的生长抑制作用,并在一定范围内呈量效关系。电镜下可见细胞膜表面微绒毛减少、染色质固缩、边集,凋亡小体形成。流式细胞术结果显示,G0/G1期的细胞比例有不同程度的增高,相应的S期细胞比例显著下降(P<0.01);而lovo细胞的细胞周期时相比例无明显改变。结论SFPS在体外能够显著诱导lovo和RKO细胞凋亡,这可能是SFPS抑制人大肠癌细胞增殖的机制之一。

The in vitro apoptosis of human colon cancer cells induced by SFPS

Objective To study the anti-tumor effects of SFPS against human colon cancer cells.Methods Inhibition of the cell proliferation was measured by MTT assay.SFPS induced apoptosis of lovo and RKO cells was observed by electron microscopy and flow cytometry.The potential of SFPS in inhibiting lovo and RKO cells viability was assessed by MTT assay.Results SFPS significantly exhibited antiproliferative activity which depended on dosage.Morphological examination showed chromosomal condensation, karyotheca margination,cell shrinkage and the presence of apoptosis bodies.The overall effect of SFPS on the cell cycle distribution was examined by flow cytometry.However,it was also found that SFPS arrested the human colon cancer cell line RKO at G0/G1 phase,and the RKO cells at S phase decreased significantly,while no change in cell cycle distribution from SFPS treated lovo cells was observed.Conclusion SFPS may induce the apoptosis of lovo and RKO cells in vitro through anti-tumor proliferation.