新生鼠脑皮层星形胶质细胞培养及鉴定

目的：旨在获得较纯的星型胶质细胞，为进一步研究奠定基础。方法：采用细胞分离培养一差速粘附法。胰酶消化及机械吹打，分离SD新生1周大鼠大脑皮层细胞，用含20％血清DMEM／F12培养基培养。并以GFAP-FITC免疫荧光法对所获细胞进行鉴定。结果：经原代及传代培养，获得大量几乎为单一种类细胞，其特点为胞体较大，形状不规则，胞质较丰富，细胞核圆形或卵圆形，常偏于胞体一侧，细胞突起较多较长，符合星形胶质细胞形态。GFAP-FITC免疫荧光染色绝大多数细胞为强阳性。结论：细胞分离培养-差速粘附法可获得较纯的星形胶质细胞。

Culture and identification of astrocytes from the cortical tissue of new rat brain in vitro

Objective:To obtain the more purified astrocytes to settle background for further study.Methods:The cortical tissue of SD rat brain isolated was digested with 0.125% trypsin and cultured with DMEM/F12 medium containing 20% blood serum. Growth condition of the cells was observed under reversed microscope every day. The derivation of the cells was identified by immunohistochemical staining with anti-mouse GFAP.Result:Almost pure cells were obtained after primary culture and subculture. The cell showed unregulated shapes, big cell body, abundant cytoplasm, many longer apophyses and round or ovi-round karyons which leaned to one side. It coincided with the form of astrocytes. Immunohistochemical staining demonstrated that nearly all of the cells were positive.Conclusion:More purified astrocytes can be obtained by this method.